AB171738

重组Anti-Superoxide Dismutase 3/EC-SOD抗体[EPR10860]

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860]

RabMAb
Recombinant
了解详情

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(8 Publications)

Rabbit Recombinant Monoclonal Superoxide Dismutase 3/EC-SOD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

查看别名

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (AB171738)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (AB171738)

Immunohistochemistry of paraffin-embedded Human heart tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (AB171738)

Immunohistochemistry of paraffin-embedded Human kidney tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (AB171738)

All lanes:

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (ab171738) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Predicted band size: 26 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10860

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 4:101975 PubMed36580401

2022

In utero intracerebroventricular delivery of adeno-associated viral vectors to target mouse choroid plexus and cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Ahram Jang,Maria K Lehtinen

Neuron 110:3288-3301.e8 PubMed36070751

2022

Choroid plexus-CSF-targeted antioxidant therapy protects the brain from toxicity of cancer chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahram Jang,Boryana Petrova,Taek-Chin Cheong,Miriam E Zawadzki,Jill K Jones,Andrew J Culhane,Frederick B Shipley,Roberto Chiarle,Eric T Wong,Naama Kanarek,Maria K Lehtinen

BMC complementary medicine and therapies 22:180 PubMed35799227

2022

Oridonin attenuates low shear stress-induced endothelial cell dysfunction and oxidative stress by activating the nuclear factor erythroid 2-related factor 2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Chen,Heqian Liu,Xiaoqi Zhao,Subinur Mamateli,Cheng Liu,Lei Wang,Jing Yu,Yutong Liu,Jing Cai,Tong Qiao

Antioxidants (Basel, Switzerland) 11: PubMed35624792

2022

SOD3 Suppresses the Expression of MMP-1 and Increases the Integrity of Extracellular Matrix in Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hyung Kim,Hae Dong Jeong,Min Ji Song,Dong Hun Lee,Jin Ho Chung,Seung-Taek Lee

Bone research 10:38 PubMed35477573

2022

A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yishan Chen,Yeke Yu,Ya Wen,Juan Chen,Junxin Lin,Zixuan Sheng,Wenyan Zhou,Heng Sun,Chengrui An,Jiansong Chen,Weiliang Wu,Chong Teng,Wei Wei,Hongwei Ouyang

Bioengineered 12:7213-7222 PubMed34558381

2021

Knockdown of Tripartite motif-containing 22 (TRIM22)relieved the apoptosis of lens epithelial cells by suppressing the expression of TNF receptor-associated factor 6 (TRAF6).

Applications

Unspecified application

Species

Unspecified reactive species

Kai Meng,Chengbo Fang

Oxidative medicine and cellular longevity 2017:7094781 PubMed28642812

2017

The Antioxidant Machinery of Young and Senescent Human Umbilical Vein Endothelial Cells and Their Microvesicles.

Applications

Species

Human

Guillermo Bodega,Matilde Alique,Lourdes Bohórquez,Sergio Ciordia,María C Mena,Manuel R Ramírez

Archives of plastic surgery 40:517-21 PubMed24086803

2013

Expression of extracellular superoxide dismutase protein in diabetes.

Applications

IHC-P, WB

Species

Human, Human

Chul Han Kim

View all publications

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重组Anti-Superoxide Dismutase 3/EC-SOD抗体[EPR10860]