AB13499

Anti-Superoxide Dismutase 1抗体

Name: Superoxide Dismutase 1抗体 (ab13499)| Abcam中文官网
Brand: Abcam Limited
SKU: AB13499
Price: 4843 CNY
Availability: InStock

Anti-Superoxide Dismutase 1 antibody

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(6 Publications)

Rabbit Polyclonal Superoxide Dismutase 1 antibody. Suitable for IHC-P, WB, ICC/IF, IP, ELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Rat Sod1.

查看别名

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

ab13499 staining Human normal colon. Staining is localized to the nucleus.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Superoxide Dismutase 1 with ab13499 at 1/120 dilution. Secondary antibody : FITC Goat Anti-Rabbit (green) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunofluorescent analysis of 2% Formaldehyde-fixed Cervical cancer cell line (HeLa) labeling Superoxide Dismutase 1 with ab13499 at 1/120 dilution. Secondary antibody : APC Goat Anti-Rabbit (red) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

Unknown

Western blot - Anti-Superoxide Dismutase 1 antibody (AB13499)

All lanes:

Western blot - Anti-Superoxide Dismutase 1 antibody (ab13499) at 1 µg/mL

All lanes:

Cell lysates prepared from mixed human cell lines (A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G)

Predicted band size: 15 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ELISA, WB, ICC/IF, IP, IHC-P

applications

免疫原

Synthetic Peptide within Rat Sod1. The exact immunogen used to generate this antibody is proprietary information.

P07632

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/120", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

This antibody was purified on an antigen coupled sepharose column.

存储溶液

pH: 7 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.

Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

See full target information Sod1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21638 PubMed38062139

2023

The interplay between sex, time of day, fasting status, and their impact on cardiac mitochondrial structure, function, and dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Mariame S Kane,Gloria A Benavides,Edie Osuma,Michelle S Johnson,Helen E Collins,Yecheng He,David Westbrook,Silvio H Litovsky,Kasturi Mitra,John C Chatham,Victor Darley-Usmar,Martin E Young,Jianhua Zhang

Reproductive biology and endocrinology : RB&E 17:23 PubMed30760288

2019

Activation of Nrf2/Keap1 pathway by oral Dimethylfumarate administration alleviates oxidative stress and age-associated infertility might be delayed in the mouse ovary.

Applications

Unspecified application

Species

Unspecified reactive species

Nana Akino,Osamu Wada-Hiraike,Wataru Isono,Hiromi Terao,Harunori Honjo,Yuichiro Miyamoto,Michihiro Tanikawa,Kenbun Sone,Mana Hirano,Miyuki Harada,Tetsuya Hirata,Yasushi Hirota,Kaori Koga,Katsutoshi Oda,Tomoyuki Fujii,Yutaka Osuga

Redox biology 19:166-178 PubMed30172101

2018

Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas E Buglak,Wulin Jiang,Edward S M Bahnson

Journal of applied physiology (Bethesda, Md. : 198 125:938-946 PubMed29792553

2018

Mitochondrial complex I defect resulting from exercise-induced lower limb ischemia in patients with peripheral arterial disease.

Applications

Unspecified application

Species

Unspecified reactive species

I Signolet,P Abraham,S Chupin,M Ammi,N Gueguen,F Letournel,J Picquet,C Baufreton,M Daligault,V Procaccio,P Reynier,S Henni

Frontiers in neuroscience 10:437 PubMed27729845

2016

In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Gasperini,Elisa Meneghetti,Giuseppe Legname,Federico Benetti

Histology and histopathology 23:1477-83 PubMed18830933

2008

Decreased expression of antioxidant enzymes in the conjunctival epithelium of dry eye (Sjögren's syndrome) and its possible contribution to the development of ocular surface oxidative injuries.

Applications

IHC-Fr

Species

Human

J Cejková,T Ardan,Z Simonová,C Cejka,J Malec,D Dotrelová,B Brunová

View all publications

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Anti-Superoxide Dismutase 1抗体

Anti-Superoxide Dismutase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Immunocytochemistry/ Immunofluorescence - Anti-Superoxide Dismutase 1 antibody (AB13499)

Western blot - Anti-Superoxide Dismutase 1 antibody (AB13499)