Anti-Superoxide Dismutase 1抗体[2F5] (ab115750)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)](https://www.abcam.cn/ps/products/115/ab115750/Images/ab115750-127875-anti-superoxide-dismutase-1-antibody-2f5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)")
Key features and details
- Mouse monoclonal [2F5] to Superoxide Dismutase 1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Superoxide Dismutase 1抗体[2F5]
参阅全部 Superoxide Dismutase 1 一抗 -
描述
小鼠单克隆抗体[2F5] to Superoxide Dismutase 1 -
宿主
Mouse -
特异性
ab115750 does not cross react with immunoglobulins but does react weakly with Mn SOD -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Cow
-
免疫原
Tissue, cells or virus corresponding to Cow Superoxide Dismutase 1. Superoxide dismutase enzyme prepared from bovine erythrocytes
-
阳性对照
- Human Kidney and Human Liver tissues
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2F5 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115750于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Destroys radicals which are normally produced within the cells and which are toxic to biological systems. -
疾病相关
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. -
序列相似性
Belongs to the Cu-Zn superoxide dismutase family. -
翻译后修饰
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. -
细胞定位
Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. - Information by UniProt
-
数据库链接
- Entrez Gene: 281495 Cow
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00442 Cow
- SwissProt: P00441 Human
- Unigene: 443914 Human
-
别名
- ALS antibody
- ALS1 antibody
- Amyotrophic lateral sclerosis 1 adult antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)](https://www.abcam.cn/ps/products/115/ab115750/Images/ab115750-127874-anti-superoxide-dismutase-1-antibody-2f5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody [2F5] (ab115750)Human Liver (formalin-fixed, paraffin-embedded) stained with ab115750 at 15 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab115750 尚未被引用在任何文献中。