AB109005

重组Anti-Sumo 2 + Sumo 3抗体[EPR4602]

Name: Sumo 2 + Sumo 3抗体[EPR4602] (ab109005)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109005
Price: 3106 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Sumo 2 + Sumo 3 antibody [EPR4602]

RabMAb
Recombinant
了解详情

(3 Reviews)

(15 Publications)

Rabbit Recombinant Monoclonal Sumo 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

查看别名

SMT3B, SMT3H2, SUMO2, Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

ab109005 at 1/100 dilution staining Sumo 2+3 in Human colon by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

ab109005 at 1/100 diltuion staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

SW480 cell lysate at 10 µg

Lane 5:

HL60 cell lysate at 10 µg

Predicted band size: 12 kDa

false

Lab

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (AB109005)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Sumo 1 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-1-protein-ab140417'>ab140417</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Sumo 2 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-2-protein-ab140420'>ab140420</a>) at 0.01 µg

Lane 3:

Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg

Lane 4:

Western blot - Recombinant Human Sumo 4 protein (<a href='/products/proteins-peptides/recombinant-human-sumo-4-protein-ab157025'>ab157025</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 16 kDa

true

Exposure time: 20s

不同偶联物与剂型 (3)

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Sumo 2 + Sumo 3 antibody [EPR4602]
Carrier free

Anti-Sumo 2 + Sumo 3 antibody [EPR4602] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Sumo 2 + Sumo 3 antibody [EPR4602]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4602

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/400", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Sumo 2 and Sumo 3 are small ubiquitin-like modifier proteins involved in post-translational modification often referred to as SUMOylation. Alternately known as SUMO-2 and SUMO-3 these proteins weigh approximately 11 kDa each and are widely expressed across various tissues including the heart and brain. During stress conditions cells significantly increase the conjugation of these proteins to other cellular proteins thereby altering their functions subcellular localization or interactions.

Biological function summary

Sumo 2 and Sumo 3 play essential roles in numerous cellular processes by modifying several target proteins in the nucleus and cytoplasm. They are part of a complex regulatory system that modulates processes like nuclear transport chromosome segregation and DNA repair. Sumo 2/3 often work in tandem with other members of the SUMO family including SUMO-1 to ensure proper cellular homeostasis.

Pathways

Sumo 2 and Sumo 3 are intimately involved in fundamental cellular signaling pathways such as the p53 pathway and NF-κB pathway. These proteins modulate the activity of key proteins within these pathways including the tumor suppressor p53 and the transcription factor NF-κB which regulate cell survival proliferation and apoptosis. Sumo 2/3-related modifications can alter the stability and function of these proteins thereby influencing cellular response to stimuli.

Alterations in Sumo 2 and Sumo 3 functionality have been associated with neurodegenerative diseases and various cancers. In neurodegenerative disorders like Huntington's disease abnormal SUMOylation of specific proteins affects neuronal survival and function. Similarly in cancer dysregulation of SUMOylation pathways can affect the expression and activity of the oncogenic protein c-Myc contributing to tumor progression. These associations underline the significant role of Sumo 2/3 in pathophysiology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed : 26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed : 18408734, PubMed : 18538659, PubMed : 21965678, PubMed : 9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed : 24105744).

See full target information SUMO2

其他靶点

SUMO3

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 26:275 PubMed41024125

2025

ZNF184-mediated transcriptional activation of SAE1 drives the cell cycle entry and immune evasion in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Shi,Luquan Zhang,Chunli Wang,Qingwei Meng

Cell reports. Medicine 4:101286 PubMed37951217

2023

A combinatorial therapeutic approach to enhance FLT3-ITD AML treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Long,Xinjie Chen,Yan Shen,Yichen Lei,Lili Mu,Zhen Wang,Rufang Xiang,Wenhui Gao,Lining Wang,Ling Wang,Jieling Jiang,Wenjun Zhang,Huina Lu,Yan Dong,Yi Ding,Honghu Zhu,Dengli Hong,Yi Eve Sun,Jiong Hu,Aibin Liang

Journal of cellular physiology 237:4580-4590 PubMed36317691

2022

Age-related SUMOylation of PLK1 is essential to meiosis progression in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Mo,Feng Liu,Chun-Hua Xing,Meng-Meng Shan,Bo Yao,Qi-Qi Sun,Yuan-Jing Zou,Kun-Huan Zhang,Jun Tan,Shao-Chen Sun,Yan-Ping Ren

Kidney diseases (Basel, Switzerland) 8:424-435 PubMed36466072

2022

SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Lirui Wang,Jiangtao Li,Chen Yu

PLoS pathogens 16:e1008447 PubMed32176739

2020

STUB1 is targeted by the SUMO-interacting motif of EBNA1 to maintain Epstein-Barr Virus latency.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyan Wang,Shujuan Du,Caixia Zhu,Chong Wang,Nuoya Yu,Ziqi Lin,Jin Gan,Yi Guo,Xinxin Huang,Yuping He,Erle Robertson,Di Qu,Fang Wei,Qiliang Cai

PLoS pathogens 15:e1008174 PubMed31830143

2019

Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Ding,Qing Zhu,Feng Zhou,Hongsheng Tan,Wenjia Xu,Chengling Pan,Caixia Zhu,Yuyan Wang,Hong Zhang,Wenwei Fu,Zhikang Qian,Zhenghong Yuan,Hongxi Xu,Fang Wei,Qiliang Cai

Cell biology international 44:560-568 PubMed31642552

2019

SUMO-modified bone marrow mesenchymal stem cells promoted the repair of articular cartilage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Meng Li,Zhanhai Yin,Shuangli Zhou,Yusheng Qiu

Scientific reports 9:6477 PubMed31019221

2019

Insulin-dependent GLUT4 trafficking is not regulated by protein SUMOylation in L6 myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth E Carmichael,Kevin A Wilkinson,Tim J Craig

The international journal of neuropsychopharmacology 22:232-246 PubMed30535257

2018

Small Extracellular Vesicles in Rat Serum Contain Astrocyte-Derived Protein Biomarkers of Repetitive Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Cristóbal Gómez-Molina,Mauricio Sandoval,Roberto Henzi,Juan Pablo Ramírez,Manuel Varas-Godoy,Alejandro Luarte,Carlos Andres Lafourcade,Alejandra Lopez-Verrilli,Karl-Heinz Smalla,Thilo Kaehne,Ursula Wyneken

Bioscience reports 38: PubMed29700214

2018

Silencing SUMO2 promotes protection against degradation and apoptosis of nucleus pulposus cells through p53 signaling pathway in intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Zhong Jin,Ji-Shou Lu,Jian-Wen Gao

View all publications

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重组Anti-Sumo 2 + Sumo 3抗体[EPR4602]