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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Sumo

Anti-Sumo 2 + Sumo 3抗体(ab3742)

  • Datasheet
  • SDS
Reviews (19)Q&A (4)References (104)

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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Western blot - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)

Key features and details

  • Rabbit polyclonal to Sumo 2 + Sumo 3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Sumo 2 + Sumo 3抗体
    参阅全部 Sumo 2 + Sumo 3 一抗
  • 描述

    兔多克隆抗体to Sumo 2 + Sumo 3
  • 宿主

    Rabbit
  • 特异性

    Recognises 2 bands representing Sumo 2 and Sumo 3 at 15 and 18kDa in Hela Nuclear extract by Western blotting.


    Replenishment batches of our polyclonal antibody, ab3742 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab81371.

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Chinese hamster
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa Nuclear cell lysate. IHC-P: Human colon adenocarcinoma tissue. ICC/IF: HepG2 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3742于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (3)
1/100.
WB (14)
1/1000. Detects a band of approximately 15, 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa).
IHC-P (1)
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 15, 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性

    SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them.
  • 细胞定位

    Cytoplasmic (SUMO3) and Nuclear (SUMO2)
  • 数据库链接

    • Entrez Gene: 100689105 Chinese hamster
    • Entrez Gene: 286807 Cow
    • Entrez Gene: 6612 Human
    • Entrez Gene: 6613 Human
    • Entrez Gene: 170930 Mouse
    • Entrez Gene: 20610 Mouse
    • Entrez Gene: 397044 Pig
    • Entrez Gene: 499417 Rat
    • Entrez Gene: 690244 Rat
    • Entrez Gene: 444021 Xenopus laevis
    • Omim: 602231 Human
    • Omim: 603042 Human
    • SwissProt: Q6LDZ8 Chinese hamster
    • SwissProt: P61955 Cow
    • SwissProt: P55854 Human
    • SwissProt: P61956 Human
    • SwissProt: P61957 Mouse
    • SwissProt: Q9Z172 Mouse
    • SwissProt: P61958 Pig
    • SwissProt: P61959 Rat
    • SwissProt: Q5XIF4 Rat
    • SwissProt: Q6GPW2 Xenopus laevis
    • SwissProt: Q6DHL4 Zebrafish
    see all
  • 别名

    • HSMT3 antibody
    • MGC117191 antibody
    • OTTHUMP00000115275 antibody
    • OTTHUMP00000115276 antibody
    • OTTHUMP00000115277 antibody
    • Sentrin 2 antibody
    • Small ubiquitin like modifier 2 antibody
    • Small ubiquitin like modifier 3 antibody
    • Small ubiquitin related modifier 2 antibody
    • Small ubiquitin related modifier 3 antibody
    • small ubiquitin-like modifier 3 antibody
    • small ubiquitin-related modifier 3 antibody
    • SMT3 homolog 1 antibody
    • SMT3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 2 antibody
    • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) antibody
    • SMT3 suppressor of mif two 3 homolog 3 antibody
    • SMT3A antibody
    • SMT3B antibody
    • SMT3H1 antibody
    • SMT3H2 antibody
    • Sumo2 antibody
    • Sumo3 antibody
    • Ubiquitin like protein SMT3A antibody
    • Ubiquitin like protein SMT3B antibody
    • Ubiquitin-like protein SMT3B antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)

    ab3742 staining Sumo 2 + Sumo 3 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab3742 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image from Cuchet-Lourenço D et al., PLoS Pathog. 2011;7(7):e1002123. Fig 9(B).; doi: 10.1371/journal.ppat.1002123. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Left-hand images show uninfected cells and the co-localization of SUMO-2/3 with PML (red) in control (upper rows of each block of 4 images) and PML depleted (low rows of each block of 4 images) HepaRG cells. Right-hand images show typical examples of recruitment of the indicated proteins to sites associated with HSV-1 genomes (ICP4; red) in cells at the edges of ICP0 null mutant (ΔICP0) plaques in control and PML depleted HepaRG cells. Scale bars indicate 5 µm.

    Cells on glass coverslips were fixed with 1.5% formaldehyde in PBS containing 2% sucrose then treated with 0.5% Nonidet P40 substitute in PBS/10% sucrose. SUMO-2/3 was detected with ab3742. An Alexa-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Western blot - Anti-Sumo 2 + Sumo 3 antibody (ab3742)

    Lanes 1 & 3: Sumo 2+3 antibody (ab3742) at 1/500 dilution
    Lanes 2 & 4: Sumo 2+3 antibody (ab3742) at 1/1000 dilution
    Lanes 1-4: HeLa nuclear extract at 20 ug

    Lane 1: as above
    Lane 2: as above
    Lane 3: Sumo 2+3 peptide (ab13760) at 1 ug/ml
    Lane 4: Sumo 2+3 peptide (ab13760) at 1 ug/ml


    Secondary
    Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution developed using the ECL technique

    Performed under reducing conditions.
     
    Exposure time: 1 minute

    Predicted band sizes : 11.6 & 10.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    IHC image of Sumo 2+3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3742, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image courtesy of Human Protein Atlas

    Image courtesy of Human Protein Atlas

    ab3742 staining Sumo 2 + 3 in Human skin. The  paraffin embedded human skin tissue was incubated with ab3742 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab3742 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

    Immunofluorescent imaging of human cells (U2OS) with ab3742 confirms the specificity of this antibody.  Antibody signal is localised exclusively to the nucleus, with diffuse background staining of the nucleoplasm.  Intense foci of staining are also evident, corresponding to SUMO-2/3 accumulation in nuclear subdomains such as the PML body.  This image is in exact agreement with several published reports (see for example Saitoh H et al.). 

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are stained with Hoechst stain.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of an Abreview submitted by Andrew Lane

    ab3742 staining Sumo 2+3 in Xenopus laevis oocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP-40 0.1% in PBS and blocked with 3% BSA for 60 minutes at 23°C. Samples were incubated with primary antibody (1/250 in PBS + 3% BSA) for 1 hour at 23°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (104)

发表研究结果有使用 ab3742?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3742 被引用在 104 文献中.

  • Gu C  et al. Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose. Mol Med Rep 27:N/A (2023). PubMed: 36601740
  • Weickert P  et al. SPRTN patient variants cause global-genome DNA-protein crosslink repair defects. Nat Commun 14:352 (2023). PubMed: 36681662
  • Huang D  et al. Crosstalk between PML and p53 in response to TGF-β1: A new mechanism of cardiac fibroblast activation. Int J Biol Sci 19:994-1006 (2023). PubMed: 36778116
  • Kleijwegt C  et al. Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus. Elife 12:N/A (2023). PubMed: 37227756
  • Antoniani F  et al. Loss of PML nuclear bodies in familial amyotrophic lateral sclerosis-frontotemporal dementia. Cell Death Discov 9:248 (2023). PubMed: 37454169
View all Publications for this product

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1-10 of 23 Abreviews or Q&A

Western blot abreview for Anti-Sumo 2 + Sumo 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Treatment
DMSO and SAE1 inhibitor
Specification
U2OS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Isabelle Cristine Da Costa

Verified customer

提交于 Mar 16 2022

Immunoprecipitation abreview for Anti-Sumo 2 + Sumo 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549)
Total protein in input
4e+006 cells
Immuno-precipitation step
Protein G
Specification
A549
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 15 2021

Western blot abreview for Anti-Sumo 2 + Sumo 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
10 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Manohar Kodavati

Verified customer

提交于 Oct 30 2018

Western blot abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
1 µg
Specification
HeLa cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 29 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell)
Permeabilization
Yes - 0.1% TX100, 2 minutes
Specification
HeLa cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 29 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer PH 6 in a PT link 20 minutes at 96C
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Virginia Gutiérrez

Verified customer

提交于 Oct 10 2015

Western blot abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
HEK 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 11 2015

Western blot abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 07 2014

Western blot abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (Xenopus egg extract)
Loading amount
40 µg
Specification
Xenopus egg extract
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Apr 10 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 2+3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Nuclei generated in oocyte extract)
Specification
Nuclei generated in oocyte extract
Fixative
Formaldehyde
Permeabilization
Yes - PBS-0.1% NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

提交于 Mar 26 2013

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