Anti-Sumo 2 + Sumo 3抗体(ab3742)
Key features and details
- Rabbit polyclonal to Sumo 2 + Sumo 3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Sumo 2 + Sumo 3抗体
参阅全部 Sumo 2 + Sumo 3 一抗 -
描述
兔多克隆抗体to Sumo 2 + Sumo 3 -
宿主
Rabbit -
特异性
Recognises 2 bands representing Sumo 2 and Sumo 3 at 15 and 18kDa in Hela Nuclear extract by Western blotting. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa Nuclear cell lysate. IHC-P: Human colon adenocarcinoma tissue. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ICC/IF | (3) |
1/100.
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WB | (14) |
1/1000. Detects a band of approximately 15, 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa).
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IHC-P | (1) |
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 15, 18 kDa (predicted molecular weight: 11.6 , 10.8 kDa). |
IHC-P
1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
SUMO proteins, such as Sumo 2 and Sumo 3, post-translationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. Sumo 2 and Sumo 3 are highly homologous, hence it is very difficult to produce antibodies which distinguish them. -
细胞定位
Cytoplasmic (SUMO3) and Nuclear (SUMO2) -
数据库链接
- Entrez Gene: 100689105 Chinese hamster
- Entrez Gene: 286807 Cow
- Entrez Gene: 6612 Human
- Entrez Gene: 6613 Human
- Entrez Gene: 170930 Mouse
- Entrez Gene: 20610 Mouse
- Entrez Gene: 397044 Pig
- Entrez Gene: 499417 Rat
see all -
别名
- HSMT3 antibody
- MGC117191 antibody
- OTTHUMP00000115275 antibody
see all
图片
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ab3742 staining Sumo 2 + Sumo 3 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab3742 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image from Cuchet-Lourenço D et al., PLoS Pathog. 2011;7(7):e1002123. Fig 9(B).; doi: 10.1371/journal.ppat.1002123. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Left-hand images show uninfected cells and the co-localization of SUMO-2/3 with PML (red) in control (upper rows of each block of 4 images) and PML depleted (low rows of each block of 4 images) HepaRG cells. Right-hand images show typical examples of recruitment of the indicated proteins to sites associated with HSV-1 genomes (ICP4; red) in cells at the edges of ICP0 null mutant (ΔICP0) plaques in control and PML depleted HepaRG cells. Scale bars indicate 5 µm.
Cells on glass coverslips were fixed with 1.5% formaldehyde in PBS containing 2% sucrose then treated with 0.5% Nonidet P40 substitute in PBS/10% sucrose. SUMO-2/3 was detected with ab3742. An Alexa-conjugated anti-rabbit IgG was used as the secondary antibody.
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Lanes 1 & 3: Sumo 2+3 antibody (ab3742) at 1/500 dilution
Lanes 2 & 4: Sumo 2+3 antibody (ab3742) at 1/1000 dilution
Lanes 1-4: HeLa nuclear extract at 20 ugLane 1: as above
Lane 2: as above
Lane 3: Sumo 2+3 peptide (ab13760) at 1 ug/ml
Lane 4: Sumo 2+3 peptide (ab13760) at 1 ug/ml
Secondary
Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution developed using the ECL technique
Performed under reducing conditions.
Exposure time: 1 minutePredicted band sizes : 11.6 & 10.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)IHC image of Sumo 2+3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3742, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody (ab3742)Image courtesy of Human Protein Atlas
Image courtesy of Human Protein Atlas
ab3742 staining Sumo 2 + 3 in Human skin. The paraffin embedded human skin tissue was incubated with ab3742 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab3742 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK
Immunofluorescent imaging of human cells (U2OS) with ab3742 confirms the specificity of this antibody. Antibody signal is localised exclusively to the nucleus, with diffuse background staining of the nucleoplasm. Intense foci of staining are also evident, corresponding to SUMO-2/3 accumulation in nuclear subdomains such as the PML body. This image is in exact agreement with several published reports (see for example Saitoh H et al.).
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are stained with Hoechst stain.
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Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody (ab3742)This image is courtesy of an Abreview submitted by Andrew Lane
ab3742 staining Sumo 2+3 in Xenopus laevis oocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with NP-40 0.1% in PBS and blocked with 3% BSA for 60 minutes at 23°C. Samples were incubated with primary antibody (1/250 in PBS + 3% BSA) for 1 hour at 23°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (91)
ab3742 被引用在 91 文献中.
- Lee YH et al. A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Mov Disord 37:767-777 (2022). PubMed: 34951052
- Ergünay T et al. Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA binding. Life Sci Alliance 5:N/A (2022). PubMed: 35022246
- Lee B et al. FK506-binding protein-like and FK506-binding protein 8 regulate dual leucine zipper kinase degradation and neuronal responses to axon injury. J Biol Chem 298:101647 (2022). PubMed: 35101451
- Alvarez-Mora MI et al. Bioenergetic and Autophagic Characterization of Skin Fibroblasts from C9orf72 Patients. Antioxidants (Basel) 11:N/A (2022). PubMed: 35740026
- Boivin M et al. Translation of GGC repeat expansions into a toxic polyglycine protein in NIID defines a novel class of human genetic disorders: The polyG diseases. Neuron 109:1825-1835.e5 (2021). PubMed: 33887199