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重组RabMAb

重组Anti-Sumo 2 + Sumo 3 + Sumo 4抗体[EPR7163] (ab126606)

Submit a review Submit a question References (6)
Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
  • OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
  • Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7163] to Sumo 2 + Sumo 3 + Sumo 4
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Sumo 2 + Sumo 3 + Sumo 4抗体[EPR7163]
    参阅全部 Sumo 2 + Sumo 3 + Sumo 4 一抗

  • 描述

    兔单克隆抗体[EPR7163] to Sumo 2 + Sumo 3 + Sumo 4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Sumo 2 + Sumo 3 + Sumo 4 aa 50-150 (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region in Sumo 4 that has significant homology with Sumo 2 and Sumo 3.
    Database link: Q6EEV6

  • 阳性对照

    • HepG3, 293T and Jurkat whole cell lysate (ab7899); Human spleen tissue lysate; HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126606于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa).
ICC/IF
1/250 - 1/500.
说明
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 15 kDa (predicted molecular weight: 11 kDa).
ICC/IF
1/250 - 1/500.
应用说明
Is unsuitable for IHC-P.

图片

  • Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Flow Cytometry (Intracellular) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

    Overlay histogram showing HEK293 cells stained with ab126606 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126606, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

    ab126606 at 1/250 dilution staining Sumo 2 + Sumo 3 + Sumo 4 in HepG2 (Human liver hepatocellular carcinoma cell line) cells by immunofluorescence.

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606) at 1/5000 dilution

    Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
    Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
    Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
    Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    OI-RD Scanning - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)
    Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR7163] (ab126606)

文献 (6)

发表研究结果有使用 ab126606？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab126606 被引用在 6 文献中.

  • Gu C  et al. Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose. Mol Med Rep 27:N/A (2023). PubMed: 36601740
  • G GK  et al. Understanding the Combined Effects of High Glucose Induced Hyper-Osmotic Stress and Oxygen Tension in the Progression of Tumourigenesis: From Mechanism to Anti-Cancer Therapeutics. Cells 12:N/A (2023). PubMed: 36980166
  • Osmanovic A  et al. A SUMO4 initiator codon variant in amyotrophic lateral sclerosis reduces SUMO4 expression and alters stress granule dynamics. J Neurol 269:4863-4871 (2022). PubMed: 35503374
  • Liang JX  et al. SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells. Oncol Lett 20:225 (2020). PubMed: 32968447
  • Ma X  et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970
  • Tan F  et al. Attenuated SUMOylation of sirtuin 1 in premature neonates with bronchopulmonary dysplasia. Mol Med Rep 17:1283-1288 (2018). PubMed: 29115559

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