重组Anti-Sumo 2 + Sumo 3 + Sumo 4抗体[EPR300(2)] (ab109196)
![Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.cn/ps/products/109/ab109196/Images/ab109196-121816-anti-sumo-23-antibody-epr3002-western-blot.jpg "Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Sumo 2 + Sumo 3 + Sumo 4抗体[EPR300(2)]
参阅全部 Sumo 2 + Sumo 3 + Sumo 4 一抗 -
描述
兔单克隆抗体[EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Paraffin-embedded Human ovarian tissue HeLa and Jurkat cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR300(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
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| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
图片
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Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.cn/ps/products/109/ab109196/Images/ab109196-121815-anti-sumo-23-antibody-epr3002-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)ab109196, at 1/100, staining Sumo 2 + Sumo 3 + Sumo 4 in paraffin-embedded Human ovarian tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.cn/ps/products/109/ab109196/Images/ab109196-451126-anti-sumo-2-sumo-3-sumo-4-antibody-epr300-2-western-blot-recombinant-human.jpg)
Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/200 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
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![Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)](https://www.abcam.cn/ps/products/109/ab109196/Images/ab109196-3-benefits-of-recombinant-antibodies.png)
Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196)
数据表及文件
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SDS download
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Datasheet download
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