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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Sumo
使用敲除细胞株进行验证重组RabMAb

重组Anti-Sumo 1抗体[Y299] - ChIP Grade (ab32058)

  • Datasheet
  • SDS
Reviews (10)Q&A (7)References (98)

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ChIP - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunoprecipitation - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
  • Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y299] to Sumo 1 - ChIP Grade
  • Suitable for: Flow Cyt (Intra), ChIP, WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

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概述

  • 产品名称

    Anti-Sumo 1抗体[Y299] - ChIP Grade
    参阅全部 Sumo 1 一抗
  • 描述

    兔单克隆抗体[Y299] to Sumo 1 - ChIP Grade
  • 宿主

    Rabbit
  • 特异性

    This antibody recognises small ubiquitin-related modifier-1 (SUMO-1), also known as SMT3, Sentrin, GMP1 UBL1 and PIC1.

  • 经测试应用

    适用于: Flow Cyt (Intra), ChIP, WB, IHC-P, ICC/IF, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, A549, C6 and NIH/3T3 cell lysates. Wild-type HAP1 whole cell lysate. IHC-P: Human endometrium, lung carcinoma and bladder carcinoma tissue. Rat stomach tissue. Mouse kidney tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. ChIP: Chromatin prepared from SK-OV-3 cells. IP: NIH/3T3 cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    Y299
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Sumo 1 antibody [Y299] (ab196310)
    • Alexa Fluor® 647 Anti-Sumo 1 antibody [Y299] (ab196533)
    • Anti-Sumo 1 antibody [Y299] - BSA and Azide free (ab219724)
    • Alexa Fluor® 568 Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab312712)
    • Alexa Fluor® 750 Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab321562)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32058于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP (1)
Use 5 µg for 25 µg of chromatin.
WB (4)
1/1000 - 1/5000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (3)
1/250 - 1/500.
IP
1/20.
说明
Flow Cyt (Intra)
1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP
Use 5 µg for 25 µg of chromatin.
WB
1/1000 - 1/5000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
IP
1/20.

靶标

  • 功能

    Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.
  • 疾病相关

    Defects in SUMO1 are the cause of non-syndromic orofacial cleft type 10 (OFC10) [MIM:613705]; also called non-syndromic cleft lip with or without cleft palate 10. OFC10 is a birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Note=A chromosomal aberation involving SUMO1 is the cause of OFC10. Translocation t(2;8)(q33.1;q24.3). The breakpoint occurred in the SUMO1 gene and resulted in haploinsufficiency confirmed by protein assays.
  • 序列相似性

    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • 翻译后修饰

    Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
    Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
  • 细胞定位

    Nucleus membrane. Nucleus speckle. Cytoplasm. Recruited by BCL11A into the nuclear body.
  • Target information above from: UniProt accession P63165 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7341 Human
    • Entrez Gene: 22218 Mouse
    • Entrez Gene: 301442 Rat
    • Omim: 601912 Human
    • SwissProt: P63165 Human
    • SwissProt: P63166 Mouse
    • SwissProt: Q5I0H3 Rat
    • Unigene: 596171 Human
    • Unigene: 81424 Human
    • Unigene: 362118 Mouse
    • Unigene: 1221 Rat
    see all
  • 别名

    • DAP1 antibody
    • GAP modifying protein 1 antibody
    • GAP-modifying protein 1 antibody
    • GMP 1 antibody
    • GMP1 antibody
    • OFC10 antibody
    • PIC 1 antibody
    • PIC1 antibody
    • SENP2 antibody
    • Sentrin 1 antibody
    • Sentrin antibody
    • Small ubiquitin related modifier 1 antibody
    • Small ubiquitin-like modifier 1 antibody
    • Small ubiquitin-related modifier 1 antibody
    • SMT3 antibody
    • SMT3 homolog 3 antibody
    • SMT3 suppressor of mif two 3 homolog 1 antibody
    • SMT3, yeast, homolog 3 antibody
    • Smt3C antibody
    • SMT3H3 antibody
    • SUMO-1 antibody
    • SUMO1 antibody
    • SUMO1_HUMAN antibody
    • Ubiquitin homology domain protein PIC1 antibody
    • Ubiquitin Like 1 antibody
    • Ubiquitin like protein SMT3C antibody
    • Ubiquitin like protein UBL1 antibody
    • Ubiquitin-homology domain protein PIC1 antibody
    • Ubiquitin-like protein SMT3C antibody
    • Ubiquitin-like protein UBL1 antibody
    • UBL 1 antibody
    • UBL1 antibody
    see all

图片

  • ChIP - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    ChIP - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    Chromatin was prepared from SK-OV-3 (Human ovarian cancer cell line) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab32058 (red, and 20µl of Anti rabbit IgG sepharose beads. 5μg of rabbit normal IgG was added to the beads control (grey).  The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    ChIP was performed according to the literature (PMID: 23770046).

  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: Sumo 1 knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Empty

    Lanes 1 - 4: Merged signal (red and green). Green - ab32058 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32058 was shown to react with Sumo 1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Sumo 1 knockout samples were used. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. Samples were incubated with ab32058 and ab8245 (Mouse anti GAPDH loading control) overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab133645 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Immunoprecipitation - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunoprecipitation - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 immunoprecipitating Sumo 1. 10µg of NIH/3T3 (Mouse embryonic fibroblast) cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

    Lane 1: NIH/3T3 whole cell lysate 10ug
    Lane 2: ab32058 IP in NIH/3T3 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32058 in NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in rat stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in human bladder carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    ab32058 staining Sumo 1 in human endometrium tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    All lanes : Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)Image from Cuchet-Lourenço D et al., PLoS Pathog. 2011 Jul;7(7):e1002123. Epub 2011 Jul 14. Fig 9.; doi:10.1371/journal.ppat.1002123; July 14, 2011, PLoS Pathog 7(7): e1002123.

    Immunofluorescence analysis of ICP0-null mutant HSV-1 infected HepaRG cells, staining Sumo1 (green) with ab32058. An AlexaFluor®-conjugated goat anti-rabbit IgG was used as the seconday antibody.

  • Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Western blot - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    All lanes : Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 12 kDa
    Observed band size: 12,80 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Flow Cytometry (Intracellular) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Sumo 1 with ab32058 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    IHC of paraffin-embedded human lung carcinoma using anti-SUMO 1 (ab32058) diluted 1:250
  • Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Immunocytochemistry/ Immunofluorescence - Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

    Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using anti-SUMO 1 (ab32058) diluted 1/250.

  • Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)
    Anti-Sumo 1 antibody [Y299] - ChIP Grade (ab32058)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (98)

发表研究结果有使用 ab32058?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab32058 被引用在 98 文献中.

  • Sun B  et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
  • Yue L  et al. HypERlnc attenuates angiotensin II-induced cardiomyocyte hypertrophy via promoting SIRT1 SUMOylation-mediated activation of PGC-1α/PPARα pathway in AC16 cells. Cell Biol Int 47:1068-1080 (2023). PubMed: 36740224
  • Awoniyi LO  et al. B cell receptor-induced protein dynamics and the emerging role of SUMOylation revealed by proximity proteomics. J Cell Sci 136:N/A (2023). PubMed: 37417469
  • Mathavarajah S  et al. PML and PML-like exonucleases restrict retrotransposons in jawed vertebrates. Nucleic Acids Res 51:3185-3204 (2023). PubMed: 36912092
  • G GK  et al. Understanding the Combined Effects of High Glucose Induced Hyper-Osmotic Stress and Oxygen Tension in the Progression of Tumourigenesis: From Mechanism to Anti-Cancer Therapeutics. Cells 12:N/A (2023). PubMed: 36980166
View all Publications for this product

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1-10 of 17 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEF)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
MEF
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 17 2019

Western blot abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
5 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 30 2018

Western blot abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Below Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (head and neck cancer cell)
Gel Running Conditions
Reduced Denaturing (8% SDS page gel)
Loading amount
30 µg
Treatment
5uM DIM-C-pPhtBu for 24h
Specification
head and neck cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 24 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Permeabilization
Yes - 0.1% Triton-X
Specification
Spinal cord
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 21 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.5%
Specification
U2OS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 01 2016

Western blot abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 01 2016

Western blot abreview for Anti-Sumo 1 antibody [Y299] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblast)
Loading amount
20 µg
Specification
Human fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 20 2012

Question


Attached is the packing slip.

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Abcam community

Verified customer

Asked on Aug 14 2012

Answer

I have issued a free of charge replacement for one vial of ab32058 with the order number 1145601.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Abcam Scientific Support

回复于 Aug 14 2012

Question

I got the following back from them and I can't say I am pleased.

They are essentially justifying cutting the unit quantity in half based on lot analysis of a single endpoint ( western analysis ).

But we of course have more than this single application. Western analysis not with standing, I don't see how we can use less than half as much on a mass basisthan the previous lot
for IP and ChIP and still get the same results.


In contrast, when we sell them ourmonoclonalsthey are sold to the end user on mass basis ( I used to do the western lot analysis myself on these, but that was just a QA check).

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Abcam community

Verified customer

Asked on Aug 13 2012

Answer

Thanks for your reply.

I am sorry for the confusion. In this case I am happy to send you 1 additional vial of ab32058 free of charge to make up for the difference in mass between the vial of ab32058 you purchased 2 years ago and the vial you purchased recently.

Going forward, I would recommend confirming the antibodyconcentration, and any other pertinent product details not specifically noted on the online datasheet, before purchase.

Please provide the PO number or Abcam order reference number associated with this most recent purchase of ab32058 so I can send you this free vial.

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Abcam Scientific Support

回复于 Aug 13 2012

Question

Customer kindly called to inquire about the concentration of this product.

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Abcam community

Verified customer

Asked on Aug 08 2012

Answer

Rabbit monoclonal antibodies do get titered based on our WB testing for each lot.
In certain cases, the antibody concentration is adjusted in order to be in the acceptable
range for WB dilution factor (in this case, 1:1000 - 10,000).
We are always happy to provide lot specific concentrations upon request if the information is available.
I hope this is helpful. Please contact me again if you have any further questions.

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Abcam Scientific Support

回复于 Aug 08 2012

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