重组Anti-Sulfite oxidase抗体[EPR7618] (ab129094)
![Western blot - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)](https://www.abcam.cn/ps/products/129/ab129094/Images/ab129094-143066-anti-sulfite-oxidase-antibody-epr7618-western-blot.jpg "Western blot - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7618] to Sulfite oxidase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Sulfite oxidase抗体[EPR7618]
参阅全部 Sulfite oxidase 一抗 -
描述
兔单克隆抗体[EPR7618] to Sulfite oxidase -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human Sulfite oxidase aa 150-250.
Database link: P51687 -
阳性对照
- Fetal kidney, HepG2, and 293T cell lysates, Human liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.90 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7618 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129094于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for ICC/IF.
靶标
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通路
Energy metabolism; sulfur metabolism. -
疾病相关
Defects in SUOX are the cause of isolated sulfite oxidase deficiency (ISOD) [MIM:272300]; also known as sulfocysteinuria. ISOD is characterized by neurological abnormalities including multicystic leukoencephalopathy with brain atrophy. Patients often suffer from seizures. Often leads to death at an early age. -
序列相似性
Contains 1 cytochrome b5 heme-binding domain. -
细胞定位
Mitochondrion intermembrane space. - Information by UniProt
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数据库链接
- Entrez Gene: 6821 Human
- Entrez Gene: 81805 Rat
- Omim: 606887 Human
- SwissProt: P51687 Human
- SwissProt: Q07116 Rat
- Unigene: 558403 Human
- Unigene: 25720 Rat
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别名
- EC 1.8.3.1 antibody
- mitochondrial antibody
- Sulfite oxidase antibody
see all
图片
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Western blot - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)All lanes : Anti-Sulfite oxidase antibody [EPR7618] (ab129094) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)](https://www.abcam.cn/ps/products/129/ab129094/Images/ab129094-143065-anti-sulfite-oxidase-antibody-epr7618-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)ab129094, at a 1/50 dilution, staining Sulfite oxidase in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] (ab129094)](https://www.abcam.cn/ps/products/129/ab129094/Images/ab129094-143064-anti-sulfite-oxidase-antibody-epr7618.jpg)
OI-RD Scanning - Anti-Sulfite oxidase antibody [EPR7618] (ab129094) -
![Anti-Sulfite oxidase antibody [EPR7618] (ab129094)](https://www.abcam.cn/ps/products/129/ab129094/Images/ab129094-4-benefits-of-recombinant-antibodies.png)
Anti-Sulfite oxidase antibody [EPR7618] (ab129094)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab129094 被引用在 4 文献中.
- Kaczmarek AT et al. A defect in molybdenum cofactor binding causes an attenuated form of sulfite oxidase deficiency. J Inherit Metab Dis 45:169-182 (2022). PubMed: 34741542
- Mellis AT et al. The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and H2S metabolism. Redox Biol 38:101800 (2021). PubMed: 33271457
- Bender D et al. Oxygen and nitrite reduction by heme-deficient sulphite oxidase in a patient with mild sulphite oxidase deficiency. J Inherit Metab Dis 43:748-757 (2020). PubMed: 31950508
- Ekman M et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). PubMed: 24589856