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Anti-Sulfatase 2/SULF2抗体[2B4] (ab113405)

Reviews (1) Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody [2B4] (ab113405)

    Key features and details

    • Mouse monoclonal [2B4] to Sulfatase 2/SULF2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Sulfatase 2/SULF2抗体[2B4]
      参阅全部 Sulfatase 2/SULF2 一抗

    • 描述

      小鼠单克隆抗体[2B4] to Sulfatase 2/SULF2

    • 宿主

      Mouse

    • 特异性

      ab113405 shows no cross-reactivity with Sulfatase 1

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Recombinant full length protein corresponding to Human Sulfatase 2/SULF2.

    • 表位

      ab113405 recognises an epitope located in the C terminal domain of Sulfatase 2/SULF2.

    • 阳性对照

      • Sulfatase 2/SULF2 transfected HEK29 cells or non-small-cell Human lung carcinoma tissue

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab113405于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    说明
    IHC-P
    1/25. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    靶标

    • 功能

      Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.

    • 组织特异性

      Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.

    • 序列相似性

      Belongs to the sulfatase family.

    • 翻译后修饰

      The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

    • 细胞定位

      Endoplasmic reticulum. Golgi apparatus > Golgi stack. Cell surface. Also localized on the cell surface.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Extracellular sulfatase Sulf 2 antibody
        • Extracellular sulfatase Sulf-2 antibody
        • HSulf-2 antibody
        • HSulf2 antibody
        • Sulf2 antibody
        • SULF2_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody [2B4] (ab113405)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sulfatase 2/SULF2 antibody [2B4] (ab113405)

        Paraffin embedded HEK293 cells transfected with Sulfatase 2/SULF2 stained with ab113405.

      文献 (4)

      发表研究结果有使用 ab113405？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab113405 被引用在 4 文献中.

      • Saraswat D  et al. Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination. Nat Commun 12:1923 (2021). PubMed: 33772011
      • Gilhooley MJ  et al. ON-bipolar cell gene expression during retinal degeneration: Implications for optogenetic visual restoration. Exp Eye Res 207:108553 (2021). PubMed: 33811915
      • Zhang L  et al. The upregulation of miR-204-3p in LPS-induced acute lung injury aggravated pulmonary endothelial cells apoptosis via targeting sulfatase 2. Acta Biochim Pol 68:217-222 (2021). PubMed: 34033710
      • Lemjabbar-Alaoui H  et al. Sulf-2, a heparan sulfate endosulfatase, promotes human lung carcinogenesis. Oncogene 29:635-46 (2010). PubMed: 19855436

      客户评价及客户问答

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Sulfatase 2/SULF2 antibody [2B4]

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK 293T Transfected with SULF2 over expressing pl)
      Permeabilization
      Yes - .1% Triton x-100 in 1XPBS with 1 % Goat Serum
      Specification
      HEK 293T Transfected with SULF2 over expressing pl
      Blocking step
      5% Goat serum in 1XPBS with 0.01% Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 30 2015

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