Anti-Substance P抗体[SP-DE4-21] (ab14184)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.cn/ps/products/14/ab14184/Images/ab14184-240737-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)")
Key features and details
- Mouse monoclonal [SP-DE4-21] to Substance P
- Suitable for: ELISA, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG1
概述
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产品名称
Anti-Substance P抗体[SP-DE4-21]
参阅全部 Substance P 一抗 -
描述
小鼠单克隆抗体[SP-DE4-21] to Substance P -
宿主
Mouse -
特异性
The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin). -
经测试应用
适用于: ELISA, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Guinea pig, Human
预测可用于: Horse, Chicken, Hamster, Cow, Shark, Common marmoset
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免疫原
Synthetic peptide corresponding to Human Substance P aa 58-68.
Sequence:RPKPQQFFGLM
Database link: P20366 -
阳性对照
- Guinea pig bone marrow. The antibody binds to the substantia gelatinosa.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
SP-DE4-21 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (12) |
1/1000 - 1/2000.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
1/1000 - 1/2000. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. -
序列相似性
Belongs to the tachykinin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 281512 Cow
- Entrez Gene: 6863 Human
- Entrez Gene: 21333 Mouse
- Entrez Gene: 24806 Rat
- Omim: 162320 Human
- SwissProt: P19850 Chicken
- SwissProt: P01289 Cow
- SwissProt: P67932 Guinea pig
see all -
别名
- C-terminal-flanking peptide antibody
- Hs.2563 antibody
- neurokinin 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)This image is courtesy of an Abreview submitted by Carl Hobbs
ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.cn/ps/products/14/ab14184/Images/ab14184-21716-anti-substance-p-antibody-sp-de4-21-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (ab14184)ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.cn/ps/products/14/ab14184/Images/ab14184-21715-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Image courtesy of an anonymous Abreview.ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)](https://www.abcam.cn/ps/products/14/ab14184/Images/ab14184-21717-anti-substance-p-antibody-sp-de4-21-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (ab14184)Substance P staining at 1:1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.
数据表及文件
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Datasheet download
文献 (45)
ab14184 被引用在 45 文献中.
- Grunz EA et al. Adventitial macrophage accumulation impairs perivascular nerve function in mesenteric arteries with inflammatory bowel disease. Front Physiol 14:1198066 (2023). PubMed: 37342800
- Lai A et al. Spinal Cord Sensitization and Spinal Inflammation from an In Vivo Rat Endplate Injury Associated with Painful Intervertebral Disc Degeneration. Int J Mol Sci 24:N/A (2023). PubMed: 36834838
- Choi JG et al. Inhibition of angiotensin converting enzyme increases PKCβI isoform expression via activation of substance P and bradykinin receptors in cultured astrocytes of mice. J Vet Sci 24:e26 (2023). PubMed: 37012034
- Nakanishi S et al. Histological study on regional specificity of the mucosal nerve network in the rat large intestine. J Vet Med Sci 85:123-134 (2023). PubMed: 36517005
- Bhadouria N et al. Raloxifene Stimulates Estrogen Signaling to Protect Against Age- and Sex-Related Intervertebral Disc Degeneration in Mice. Front Bioeng Biotechnol 10:924918 (2022). PubMed: 36032728
