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AB14184

Anti-Substance P 抗体 [SP-DE4-21]

Anti-Substance P antibody [SP-DE4-21]

4

(15 Reviews)

|

(65 Publications)

Anti-Substance P antibody [SP-DE4-21] (ab14184) is a mouse monoclonal antibody detecting Substance P in IHC-P, ICC/IF, ELISA. Suitable for Guinea pig, Human, Mouse, Rat.

- Over 40 publications
- Trusted since 2004

查看别名

NKA, NKNA, TAC2, TAC1, Protachykinin-1, PPT

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)
  • IHC-P

AbReview45827****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)

ab14184 staining Substance P in mouse spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)
  • IHC-P

AbReview28462****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)

ab14184 staining Substance P in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then incubated with ab14184 at a 1/100 dilution for 1 hour. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

Image courtesy of an anonymous Abreview.

Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (AB14184)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Substance P antibody [SP-DE4-21] (AB14184)

ICC/IF image of ab14184 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14184, 1ug/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Substance P antibody [SP-DE4-21] (AB14184)

Substance P staining at 1 : 1000 of the substancia gelatinosa (dorsal horn) of the guinea pig spinal cord using an HRP-conjugated secondary antibody and DAB.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

SP-DE4-21

亚型

IgG1

不含载体蛋白

No

反应种属

Rat, Guinea pig, Human, Mouse

应用

IHC-P, ICC/IF, ELISA

applications

免疫原

Synthetic Peptide within Human TAC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P20366

特异性

The antibody reacts specifically with human Substance P. No crossreactivity with related tachykinins (human Neurokinin A, Neurokinin B, and Kassinin).

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反应性数据

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产品详情

What is this antibody validated in?
Anti-Substance P antibody [SP-DE4-21] (ab14184) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Guinea pig, Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-Substance P [SP-DE4-21] (ab14184) was first used in a scientific publication in 2004 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Substance P [SP-DE4-21] (ab14184) has over 10 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.
Biological function summary

This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.

Pathways

Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.

Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.. Substance P. Is a ligand for TACR1, and triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding to TACR1 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity). Is also a TACR3 agonist with low receptor affinity (PubMed : 37391393). Basic secretagogue neuropeptide released from the terminals of specific sensory nerves (PubMed : 30686732). Acts as a ligand for MRGPRX2 receptor in mast cells, initiating a signaling that mediates neurogenic inflammation and pain (PubMed : 30686732, PubMed : 34789875). Neurogenic inflammation includes mast cell activation, recruitment of immune cells and release of inflammatory mediators, such as cytokines and chemokines (By similarity). The inflammatory response can then activate or sensitize nociceptors, promoting pain (By similarity).. Neurokinin A. Is a ligand for TACR2, and triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol hydrolysis by phospholipase C (PubMed : 35882833). Binding to TACR2 also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP).
See full target information TAC1
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文献 (65)

Recent publications for all applications. Explore the full list and refine your search

Journal of veterinary research 69:249-255 PubMed40552032

2025

Chemical coding of piglets small intestine neurons after prenatal exposure to β-hydroxy-β-methylbutyrate.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Dajnowska,Katarzyna Kras,Ewa Tomaszewska,Piotr Dobrowolski,Renata Klebaniuk,Siemowit Muszyński,Marcin Bartłomiej Arciszewski

Nature metabolism 7:1223-1245 PubMed40229448

2025

Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Nadja Ahrentløv,Olga Kubrak,Mette Lassen,Alina Malita,Takashi Koyama,Amalie S Frederiksen,Casper M Sigvardsen,Alphy John,Pernille E H Madsen,Kenneth V Halberg,Stanislav Nagy,Cordelia Imig,Erik A Richter,Michael J Texada,Kim Rewitz

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404835 PubMed39985258

2025

A Silk Fibroin Nanoparticle Hydrogel Loaded With NK1R Antagonist Has Synergistic Anti-Inflammatory and Reparative Effects on Dry Eye Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Gong,Yi Liu,Huan Li,Xueming Ju,Dongfeng Li,Yuhao Zou,Xiaoxin Guo,Kai Dong,Jialing Xiao,Weijia Wu,Renjie Chai,Ruifan Zhang,Man Yu

Journal of veterinary research 68:633-642 PubMed39776677

2025

The effect of prenatal fumonisin B exposure on bone innervation in newborn Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Tomaszewska,Piotr Dobrowolski,Aleksandra Dajnowska,Liwia Arbatowska,Iwona Puzio,Halyna Rudyk,Oksana Brezvyn,Ihor Kotsyumbas,Janine Donaldson,Jadwiga Śliwa,Marcin B Arciszewski,Siemowit Muszyński

Neuroendocrinology 115:269-282 PubMed39662068

2024

Immunohistochemical Identification of Sensory Neuropeptides Calcitonin Gene-Related Peptide, Substance P, and Pituitary Adenylate Cyclase-Activating Polypeptide in Efferent Vestibular Nucleus Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

David Lorincz,Hannah Rose Drury,Rebecca Lim,Alan Martin Brichta

Journal of microbiology and biotechnology 34:1769-1777 PubMed39187454

2024

The Gut Microbial Lipid Metabolite 14(15)-EpETE Inhibits Substance P Release by Targeting GCG/PKA Signaling to Relieve Cisplatin-Induced Nausea and Vomiting in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Man Lu,Liwei Xie,Sijie Yin,Jing Zhou,Lingmei Yi,Ling Ye

Bioengineering (Basel, Switzerland) 11: PubMed38790364

2024

Effect of Exposure to Particulate Matter on the Ocular Surface in an Experimental Allergic Eye Disease Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Basanta Bhujel,Seheon Oh,Woojune Hur,Seorin Lee,Ho Seok Chung,Hun Lee,Jin Hyoung Park,Jae Yong Kim

International journal of molecular sciences 25: PubMed38612517

2024

Differences in Neuropathology between Nitroglycerin-Induced Mouse Models of Episodic and Chronic Migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Songyi Park,Harry Jung,Sang-Won Han,Sang-Hwa Lee,Jong-Hee Sohn

International journal of molecular sciences 25: PubMed38339040

2024

Annulus Fibrosus Injury Induces Acute Neuroinflammation and Chronic Glial Response in Dorsal Root Ganglion and Spinal Cord-An In Vivo Rat Discogenic Pain Model.

Applications

Unspecified application

Species

Unspecified reactive species

Alon Lai,Denise Iliff,Kashaf Zaheer,Jennifer Gansau,Damien M Laudier,Venetia Zachariou,James C Iatridis

Acta pharmacologica Sinica 45:945-958 PubMed38326624

2024

Glutamatergic neurons in ventral pallidum modulate heroin addiction via epithalamic innervation in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Song Chen,Jing Liu,Yu-Jun Wang,Kuan Ning,Jing-Gen Liu,Zhi-Qiang Liu
View all publications
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