重组Anti-STUB1/CHIP抗体[EPR4447] - BSA and Azide free (ab238966)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4447] to STUB1/CHIP - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-STUB1/CHIP抗体[EPR4447] - BSA and Azide free
参阅全部 STUB1/CHIP 一抗 -
描述
兔单克隆抗体[EPR4447] to STUB1/CHIP - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human skeletal muscle tissue.
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常规说明
ab238966 is the carrier-free version of ab134064.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4447 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-STUB1/CHIP antibody [EPR4447] (ab134064)
- PE Anti-STUB1 / CHIP antibody [EPR4447] (ab303131)
- APC Anti-STUB1 / CHIP antibody [EPR4447] (ab303132)
- HRP Anti-STUB1 / CHIP antibody [EPR4447] (ab303133)
- Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447] (ab309692)
- Alexa Fluor® 647 Anti-STUB1 / CHIP antibody [EPR4447] (ab310058)
- Alexa Fluor® 594 Anti-STUB1 / CHIP antibody [EPR4447] (ab310450)
- Alexa Fluor® 555 Anti-STUB1 / CHIP antibody [EPR4447] (ab311978)
- Alexa Fluor® 568 Anti-STUB1 / CHIP antibody [EPR4447] (ab312452)
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab238966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/100.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
1/100. |
靶标
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功能
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
组织特异性
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 3 TPR repeats.
Contains 1 U-box domain. -
结构域
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10273 Human
- Entrez Gene: 56424 Mouse
- Entrez Gene: 287155 Rat
- Omim: 607207 Human
- SwissProt: Q9UNE7 Human
- SwissProt: Q9WUD1 Mouse
- Unigene: 592081 Human
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别名
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
图片
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Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling STUB1/CHIP with purified ab134064 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).
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ab134064 (purified) at 1/120 immunoprecipitating STUB1/CHIP in 10 μg HeLa (Lanes 1 and 2, observed at 35 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).
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Immunofluorescence staining of SH-SY5Y cells with purified ab134064 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab134064 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134064).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
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