AB249940

重组Anti-Striatin 4抗体[EPR11801] - BSA and Azide free

Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Striatin 4 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

ZIN, STRN4, Striatin-4, Zinedin

4 Images

Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

This data was developed using ab177155, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Striatin 4 with ab177155 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

This data was developed using ab177155, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of U87-MG cells labeling Striatin 4 with ab177155 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

This data was developed using ab177155, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate, immunoprecipitated using ab177155 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] (<a href='/products/primary-antibodies/striatin-4-antibody-epr11801-ab177155'>ab177155</a>)

Predicted band size: 80 kDa

false

Supplier Data

Western blot - Anti-Striatin 4 antibody [EPR11801] - BSA and Azide free (AB249940)

This data was developed using ab177155, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Striatin 4 antibody [EPR11801] (<a href='/products/primary-antibodies/striatin-4-antibody-epr11801-ab177155'>ab177155</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

U87-MG cell lysate at 10 µg

Lane 4:

Human cerebellum tissue lysate at 10 µg

Lane 5:

Human fetal lung tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 80 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-Striatin 4 antibody [EPR11801]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11801

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, ICC/IF, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249940 is the carrier-free version of ab177155.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Striatin 4 also known as SG2NA or STRN4 is a scaffold protein with a molecular mass of approximately 100 kDa. It is part of the striatin family and plays an important role in the organization of multiprotein complexes. The protein is expressed in several tissues including the brain where it influences signaling pathways. Striatin 4 interacts with calmodulin and phosphatases facilitating communication and modification of signaling proteins.

Biological function summary

Striatin 4 facilitates interactions within the striatin family interactosome and contributes to the modulation of calcium signaling through its role in calcium/calmodulin-dependent processes. As a regulatory component it is essential within protein complexes that have wide implications in cellular signaling. The protein supports roles in cell growth apoptosis and vascular function due to its ability to bring together various signaling proteins and enzymes impacting cellular behavior.

Pathways

Researchers have identified Striatin 4 as a participant in important pathways that include the protein phosphatase 2A (PP2A) pathway and the calcium signaling pathway. Its interactions with PP2A-related proteins suggest it may serve to regulate protein dephosphorylation events thereby affecting many downstream signaling cascades. Through its participation in calcium signaling Striatin 4 works in conjunction with calcium-binding proteins influencing various physiological processes such as muscle contraction and neurotransmitter release.

Studies have linked Striatin 4 to conditions such as Alzheimer's disease and cardiovascular disorders where its role in signaling pathways may contribute to pathological changes. Its association with proteins like tau in Alzheimer's reflects its potential influence on neurodegenerative processes. Additionally the interaction of Striatin 4 with vascular-related proteins could implicate it in the progression of cardiovascular conditions making it a target of interest for disease understanding and therapeutic development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Calmodulin-binding scaffolding protein which is the center of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 18782753, PubMed : 32640226). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling (PubMed : 32640226). Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (Probable). Key regulator of the expanded Hippo signaling pathway by interacting and allowing the inhibition of MAP4K kinases by the STRIPAK complex (PubMed : 32640226).

See full target information STRN4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong

View all publications

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-Striatin 4抗体[EPR11801] - BSA and Azide free