重组Anti-Striatin 4抗体[EPR11801] (ab177155)
![Western blot - Anti-Striatin 4 antibody [EPR11801] (ab177155)](https://www.abcam.cn/ps/products/177/ab177155/Images/ab177155-205158-anti-striatin-4-antibody-epr11801-western-blot.jpg "Western blot - Anti-Striatin 4 antibody [EPR11801] (ab177155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11801] to Striatin 4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Striatin 4抗体[EPR11801]
参阅全部 Striatin 4 一抗 -
描述
兔单克隆抗体[EPR11801] to Striatin 4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Striatin 4.
Database link: Q9NRL3 -
阳性对照
- Human fetal brain, fetal lung, and Human cerebellum lysates. K562 and U87-MG cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11801 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
|
|
| ICC/IF |
1/50 - 1/100.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa). |
|
ICC/IF
1/50 - 1/100. |
|
IP
1/10 - 1/100. |
靶标
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功能
Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein. -
序列相似性
Belongs to the WD repeat striatin family.
Contains 7 WD repeats. -
细胞定位
Cytoplasm. Membrane. Cell projection, dendritic spine. CTTNBP2-binding may regulate dendritic spine distribution. - Information by UniProt
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数据库链接
- Entrez Gene: 29888 Human
- Omim: 614767 Human
- SwissProt: Q9NRL3 Human
- Unigene: 631590 Human
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别名
- PPP2R6C antibody
- Protein phosphatase 2 regulatory subunit B'''gamma antibody
- Striatin, calmodulin binding protein 4 antibody
see all
图片
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Western blot - Anti-Striatin 4 antibody [EPR11801] (ab177155)All lanes : Anti-Striatin 4 antibody [EPR11801] (ab177155) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : Human cerebellum tissue lysate
Lane 5 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] (ab177155)](https://www.abcam.cn/ps/products/177/ab177155/Images/ab177155-205159-anti-striatin-4-antibody-epr11801-immunofluorescence.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Striatin 4 antibody [EPR11801] (ab177155)Immunofluorescent analysis of U87-MG cells labeling Striatin 4 with ab177155 at 1/50 dilution.
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![Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] (ab177155)](https://www.abcam.cn/ps/products/177/ab177155/Images/ab177155-205161-anti-striatin-4-antibody-epr11801-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Striatin 4 antibody [EPR11801] (ab177155)Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate, immunoprecipitated using ab177155 at 1/10 dilution.
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![Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] (ab177155)](https://www.abcam.cn/ps/products/177/ab177155/Images/ab177155-205160-anti-striatin-4-antibody-epr11801-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Striatin 4 antibody [EPR11801] (ab177155)Intracellular flow cytometric analysis of permeabilized K562cells labeling Striatin 4with ab177155 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
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![Anti-Striatin 4 antibody [EPR11801] (ab177155)](https://www.abcam.cn/ps/products/177/ab177155/Images/ab177155-5-benefits-of-recombinant-antibodies.png)
Anti-Striatin 4 antibody [EPR11801] (ab177155)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab177155 被引用在 3 文献中.
- Qi S et al. Two Hippo signaling modules orchestrate liver size and tumorigenesis. EMBO J 42:e112126 (2023). PubMed: 36919851
- Kim JW et al. STRIPAK directs PP2A activity toward MAP4K4 to promote oncogenic transformation of human cells. Elife 9:N/A (2020). PubMed: 31913126
- Tang Y et al. Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer. Cancer Cell 38:115-128.e9 (2020). PubMed: 32589942