重组Anti-Strep-tag II抗体[EPR12666] (ab180957)
![Western blot - Anti-Strep-tag II antibody [EPR12666] (ab180957)](https://www.abcam.cn/ps/products/180/ab180957/Images/ab180957-212260-anti-strep-tag-ii-antibody-epr12666-western-blot.jpg "Western blot - Anti-Strep-tag II antibody [EPR12666] (ab180957)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12666] to Strep-tag II
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
概述
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产品名称
Anti-Strep-tag II抗体[EPR12666]
参阅全部 Strep-tag II 一抗 -
描述
兔单克隆抗体[EPR12666] to Strep-tag II -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Strep-tag II protein
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12666 -
同种型
IgG
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180957于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 16-100 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 16-100 kDa. |
靶标
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相关性
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II. -
别名
- anti-Strep-Tag II antibody
- Strep-Tag II antibody
- Strep-Tag II antibody antibody
- Strep-Tag IIantiboy antibody
图片
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Western blot - Anti-Strep-tag II antibody [EPR12666] (ab180957)All lanes : Anti-Strep-tag II antibody [EPR12666] (ab180957) at 1/1000 dilution (Unpurified)
Lane 1 : Strep-tag II protein at 0.01 µg
Lane 2 : Strep-tag II protein at 0.005 µg
Lane 3 : Strep-tag II protein at 0.001 µg
Lane 4 : Strep-tag II protein at 0.0005 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution -
![Anti-Strep-tag II antibody [EPR12666] (ab180957)](https://www.abcam.cn/ps/products/180/ab180957/Images/ab180957-2-benefits-of-recombinant-antibodies.png)
Anti-Strep-tag II antibody [EPR12666] (ab180957)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab180957 被引用在 9 文献中.
- Seidel M et al. Co-translational binding of importins to nascent proteins. Nat Commun 14:3418 (2023). PubMed: 37296145
- Esposito E et al. Mitotic checkpoint gene expression is tuned by codon usage bias. EMBO J 41:e107896 (2022). PubMed: 35811551
- Li L et al. A highly efficient indirect ELISA and monoclonal antibody established against African swine fever virus pK205R. Front Immunol 13:1103166 (2022). PubMed: 36700212
- Kondo T et al. The BcsD subunit of type I bacterial cellulose synthase interacts dynamically with the BcsAB catalytic core complex. FEBS Lett 596:3069-3086 (2022). PubMed: 36103154
- Li L et al. The Indirect ELISA and Monoclonal Antibody against African Swine Fever Virus p17 Revealed Efficient Detection and Application Prospects. Viruses 15:N/A (2022). PubMed: 36680090
- De Caluwé L et al. The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells. Front Microbiol 12:615165 (2021). PubMed: 33717005
- Tian M et al. GluN2A and GluN2B NMDA receptors use distinct allosteric routes. Nat Commun 12:4709 (2021). PubMed: 34354080
- Nesterenko PA et al. HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses. Cell Rep 37:110167 (2021). PubMed: 34919800
- Gutierrez-Escribano P et al. A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules. Sci Adv 5:eaay6804 (2019). PubMed: 31807710