Anti-Stra8抗体(ab49405)
Key features and details
- Rabbit polyclonal to Stra8
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Stra8抗体
参阅全部 Stra8 一抗 -
描述
兔多克隆抗体to Stra8 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Stra8 (mouse) transient overexpression lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 0.1 µg/ml. Recommended use at a low concentration otherwise high background staining may be observed |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. -
组织特异性
Expressed specifically in testis. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 20899 Mouse
- SwissProt: P70278 Mouse
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别名
- Stimulated by retinoic acid 8 antibody
- stimulated by retinoic acid 8 homolog antibody
- Stimulated by retinoic acid Gene 8 antibody
see all
图片
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Western blot - Anti-Stra8 antibody (ab49405)All lanes : Anti-Stra8 antibody (ab49405) at 1 µg/ml
Lane 1 : CHO overexpressing Mouse Stra8 lysate
Lane 2 : CHO Negative Control Lysate
Lysates/proteins at 1 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteAb49405 recognizes tagged overexpressed Stra8 protein which has an expected molecular weight of 60 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stra8 antibody (ab49405)IHC image of Stra8 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49405, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (37)
ab49405 被引用在 37 文献中.
- Song Y et al. Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis. Life Sci Alliance 6:N/A (2023). PubMed: 36414375
- Nagirnaja L et al. Diverse monogenic subforms of human spermatogenic failure. Nat Commun 13:7953 (2022). PubMed: 36572685
- Liu W et al. A Novel Meiosis-Related lncRNA, Rbakdn, Contributes to Spermatogenesis by Stabilizing Ptbp2. Front Genet 12:752495 (2021). PubMed: 34707642
- Dard R et al. DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis. Genes (Basel) 12:N/A (2021). PubMed: 34828406
- Desimio MG et al. Stimulated by retinoic acid gene 8 (STRA8) interacts with the germ cell specific bHLH factor SOHLH1 and represses c-KIT expression in vitro. J Cell Mol Med 25:383-396 (2021). PubMed: 33236849