Anti-STK3/MST-2抗体(ab70546)
Key features and details
- Rabbit polyclonal to STK3/MST-2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-STK3/MST-2抗体
参阅全部 STK3/MST-2 一抗 -
描述
兔多克隆抗体to STK3/MST-2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to Human STK3/MST-2 (C terminal).
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阳性对照
- Whole cell lysate from HeLa, Ramos or NIH3T3 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70546于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/15000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/15000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. -
组织特异性
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Contains 1 SARAH domain. -
细胞定位
Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 533815 Cow
- Entrez Gene: 6788 Human
- Entrez Gene: 56274 Mouse
- Entrez Gene: 65189 Rat
- Entrez Gene: 432210 Xenopus laevis
- Entrez Gene: 100036637 Xenopus tropicalis
- Entrez Gene: 324125 Zebrafish
- Omim: 605030 Human
see all -
别名
- 0610042I06Rik antibody
- EC 2.7.11.1 antibody
- FLJ90748 antibody
see all
图片
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Western blot - Anti-STK3/MST-2 antibody (ab70546)All lanes : Anti-STK3/MST-2 antibody (ab70546) at 1/10000 dilution
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody (ab70546)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling STK3/MST-2 with ab70546 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-STK3/MST-2 antibody (ab70546)1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70546 at 3ug/mg of lysate (lane 1) or a control rabbit Ig )lane 2). For the subsequent blot, 25% of the immnuoprecipitate (lanes 1 and 2) or the input (lane 3) were probed with ab70546 at 1mg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab70546 被引用在 3 文献中.
- Li Q et al. Tea polyphenols alleviate hydrogen peroxide-induced oxidative stress damage through the Mst/Nrf2 axis and the Keap1/Nrf2/HO-1 pathway in murine RAW264.7 cells. Exp Ther Med 22:1473 (2021). PubMed: 34737813
- Brandt ZJ et al. Core Hippo pathway components act as a brake on Yap and Taz in the development and maintenance of the biliary network. Development 147:N/A (2020). PubMed: 32439761
- Chen FB et al. LINC01315 Impairs microRNA-211-Dependent DLG3 Downregulation to Inhibit the Development of Oral Squamous Cell Carcinoma. Front Oncol 10:556084 (2020). PubMed: 33117688