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重组RabMAb

重组Anti-STING (phospho Y199)抗体[EPR22728-11] - BSA and Azide free (ab318182)

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Western blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
  • Dot Blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
  • Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22728-11] to STING (phospho Y199) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-STING (phospho Y199)抗体[EPR22728-11] - BSA and Azide free
    参阅全部 STING 一抗

  • 描述

    兔单克隆抗体[EPR22728-11] to STING (phospho Y199) - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Dot blotmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: 293T cells co-transfected with a human wild-type STING1 expression vector containing a DDDDK-tag and a human NTRK2 expression vector containing a Myc-tag, whole cell lysate. Dot: STING (phospho Y199) peptide a, STING (phospho Y199) peptide b.

  • 常规说明

    ab318182 is the carrier-free version of ab318181

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318182于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
Dot blot
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
Dot blot
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

  • 组织特异性

    Ubiquitously expressed.

  • 序列相似性

    Belongs to the TMEM173 family.

  • 翻译后修饰

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.

  • 细胞定位

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt

  • 数据库链接

    • 别名

      • endoplasmic reticulum IFN stimulator antibody
      • Endoplasmic reticulum interferon stimulator antibody
      • ERIS antibody
      • FLJ38577 antibody
      • hMITA antibody
      • hSTING antibody
      • Mediator of IRF3 activation antibody
      • MITA antibody
      • Mitochondrial mediator of IRF3 activation antibody
      • MPYS antibody
      • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
      • NET23 antibody
      • Stimulator of interferon genes antibody
      • Stimulator of interferon genes protein antibody
      • STING antibody
      • TM173_HUMAN antibody
      • Tmem173 antibody
      • Transmembrane protein 173 antibody
      see all

    图片

    • Western blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
      Western blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
      All lanes : Anti-STING (phospho Y199) antibody [EPR22728-11] (ab318181) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type STING1 expression vector containing a DDDDK-tag, whole cell lysate
      Lane 2 : 293T cells transfected with a human STING1 (mutation Y199F) expression vector containing a DDDDK-tag, whole cell lysate
      Lane 3 : 293T cells co-transfected with a human wild-type STING1 expression vector containing a DDDDK-tag and a human NTRK2 expression vector containing a Myc-tag, whole cell lysate
      Lane 4 : 293T cells co-transfected with a human STING1 (mutation Y199F) expression vector containing a DDDDK-tag and a human NTRK2 expression vector containing a Myc-tag, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa


      Exposure time: 92 seconds


      This data was developed using ab318181, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

      In Western blot, Anti-Myc-tag antibody staining at 1/10000 dilution.

      In Western blot, Anti-DDDDK-tag staining at 1/5000 dilution.

    • Dot Blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
      Dot Blot - Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)

      This data was developed using ab318181, the same antibody clone in a different buffer formulation.

      Dot blot analysis of STING (phospho Y199) using ab318181 at 1:1000 (0.586 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Lane 1: STING (phospho Y199) peptide a
      Lane 2: STING (phospho Y199) peptide b
      Lane 3: STING non-phospho peptide

      Exposure time: 180 seconds.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)
      Anti-STING (phospho Y199) antibody [EPR22728-11] - BSA and Azide free (ab318182)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318182？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318182 尚未被引用在任何文献中。

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