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Stem Cells Germline Stem Cells Embryonic Germ Cells

Anti-STELLAR抗体(ab19878)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (37)

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Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)
  • Immunohistochemistry (Frozen sections) - Anti-STELLAR antibody (ab19878)
  • Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)

Key features and details

  • Rabbit polyclonal to STELLAR
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG

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概述

  • 产品名称

    Anti-STELLAR抗体
    参阅全部 STELLAR 一抗
  • 描述

    兔多克隆抗体to STELLAR
  • 宿主

    Rabbit
  • 特异性

    From November 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

  • 经测试应用

    适用于: IHC-Fr, ICC/IFmore details
    不适用于: WB
  • 种属反应性

    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse STELLAR aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab23324)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human STELLAR protein (denatured) (ab134623)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab19878于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (2)
Use a concentration of 4 µg/ml.
ICC/IF (2)
Use a concentration of 5 µg/ml.
说明
IHC-Fr
Use a concentration of 4 µg/ml.
ICC/IF
Use a concentration of 5 µg/ml.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    May play a role in maintaining cell pluripotentiality.
  • 组织特异性

    Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • 发展阶段

    Expressed in fetal ovary.
  • 细胞定位

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession Q6W0C5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 73708 Mouse
    • Entrez Gene: 297576 Rat
    • SwissProt: Q8QZY3 Mouse
    • SwissProt: Q6IMK0 Rat
    • Unigene: 27982 Mouse
    • Unigene: 79056 Rat
    • 形式

      Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
    • 别名

      • Compaction associated protein 1 antibody
      • Developmental pluripotency associated 3 antibody
      • Developmental pluripotency associated protein 3 antibody
      • Developmental pluripotency-associated protein 3 antibody
      • Dppa3 antibody
      • DPPA3_HUMAN antibody
      • PGC7 antibody
      • Stella related protein antibody
      • Stella-related protein antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)
      Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)

      ab19878 staining STELLAR in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab19878 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunohistochemistry (Frozen sections) - Anti-STELLAR antibody (ab19878)
      Immunohistochemistry (Frozen sections) - Anti-STELLAR antibody (ab19878)This image is courtesy of Petra Hajkova, University of Cambridge

      The image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.

    • Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)
      Immunocytochemistry/ Immunofluorescence - Anti-STELLAR antibody (ab19878)
      ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (37)

    发表研究结果有使用 ab19878?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab19878 被引用在 37 文献中.

    • Chen Y  et al. The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting. Protein Cell 14:477-496 (2023). PubMed: 36921016
    • Yu L  et al. Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells. Methods Mol Biol 2677:269-280 (2023). PubMed: 37464248
    • Lee J  et al. Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development. Sci Rep 13:12309 (2023). PubMed: 37516749
    • Wei X  et al. PGC7 Regulates Genome-Wide DNA Methylation by Regulating ERK-Mediated Subcellular Localization of DNMT1. Int J Mol Sci 24:N/A (2023). PubMed: 36834503
    • Pei L  et al. Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate. Stem Cells Int 2022:5283615 (2022). PubMed: 35345626
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Stella antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Germline stem cells)
    Permeabilization
    Yes - 1% Triton X in PBS
    Specification
    Germline stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 21 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Stella antibody - Primordial Germ Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, embryonic day 15.5)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, embryonic day 15.5
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    提交于 Apr 06 2016

    Western blot abreview for Anti-Stella antibody - Primordial Germ Cell Marker

    Below Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Loading amount
    200000 cells
    Specification
    mouse embryonic stem cell
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 24 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-STELLAR antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (embryonic stem cell)
    Permeabilization
    No
    Specification
    embryonic stem cell
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 16°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 11 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Stella antibody - Primordial Germ Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Adult ovary)
    Specification
    Adult ovary
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    提交于 Aug 29 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Stella antibody - Primordial Germ Cell Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Marmoset adult testis)
    Specification
    Marmoset adult testis
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target retrieval solution by Dako
    Permeabilization
    Yes - Triton-X
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    提交于 Aug 09 2012

    Question

    Thank you for reply to my query.
    For the time being, I would prefer a credit note, as I have already received an aliquot of an antibody against DPPA3 from a colleague of mine.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2012

    Answer

    Thank you for contacting us.

    Your credit note ID is XXXXX.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.

    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    回复于 Oct 18 2012

    Question

    Please note that the antibody is NOT binding all over! If you look at the pictures that I sent you attached to my previous mail you will see that NOT all nuclei are positive. Especially in cartilage you see positive nuclei (stained brown) and negative nuclei (stained blue). The same you see in the intestine. Here there is stronger staining in epithelial nuclei towards the tip of the villus and in addition the nuclei in the mesenchyme are plainly negative!
    Hence, your suggestions are not valid, as the antibody binds to specific cell types, but, unfortunately, it should not do so, being supposedly a marker for primordial germ cells.

    Also, if you do antigen retrieval using Tris EDTA you still have to heat the tissue!?! And frequently cooking in a pressure cooker is recommended for AG retrieval.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Oct 17 2012

    Question

    1) Abcam product code ab19878
    2) Abcam order reference number or product batch number GR77041-1
    3) Description of the problem Nuclear staining in multiple tissues (such as skeletal muscle, epithelium of the small intestine, cartilage, brown adipose tissue, spongiotrophoblasts, and others) that should be negative for DPPA3; cytoplasmic staining in pancreas; no staining in heart muscle. This pattern was found consistently in 4 experiments on E18 and E19 mouse fetuses and placentas.
    4) Sample preparation:
    Species
    Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other: Carnoy's fixed mouse fetuses and placentas on E18 and E19 of gestation.
    Sample preparation 7 µm paraffin sections
    Positive control Fetal gonads
    Negative control Rest of fetus
    5) Fixation step
    Yes/No
    If yes: Fixative agent and concentration Carnoy's fixative (60% ethanol, 30% CHCl3, 10% acetic acid)
    Fixation time over night (10-12 h)
    Fixation temperature 4 °C
    6) Antigen retrieval method Citrate buffer, pH 6.0, 10 min at 480 W in microwave, cooling period 40-50 min
    7) Permeabilization method: None
    Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
    Permeabilizing agent and concentration:
    8) Blocking agent (eg BSA, serum…): Dako antibody dilution solution (S3022)
    Concentration
    Blocking time 30 min
    Blocking temperature RT
    9) Endogenous peroxidases blocked? 3.5% H2O2 in PBS, 10 min @ RT
    Endogenous biotins blocked? No
    10) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution Between 1 : 200 to 1 : 400 (recommended by abcam 1:250)
    Diluent buffer Dako antibody dilution medium (S3022)
    Incubation time
    11) Secondary antibody:
    Species: Swine (DAKO P0399)
    Reacts against: Rabbit
    Concentration or dilution 1 : 100
    Diluent buffer Dako antibody dilution medium (S3022)
    Incubation time 60 min at RT
    Fluorochrome or enzyme conjugate HRP
    12) Washing after primary and secondary antibodies:
    Buffer 1 x PBS
    Number of washes 3 x 5 min
    13) Detection method HRP
    14) How many times have you run this staining? 4 x
    Do you obtain the same results every time? Yes
    What steps have you altered to try and optimize the use of this antibody? Different concentrations of primary AB
    Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem. Picures show
    a-DPPA3 1/200 E19 cartl = nuclear anti-DPPA3 staining in cartilage of E19 mouse fetus (dilution of primary AB 1 : 200)
    a-DPPA3 1/400 E19 cartl = nuclear anti-DPPA3 staining in cartilage of E19 mouse fetus (dilution of primary AB 1 : 400) (dilution of primary AB 1 : 400)
    a-DPPA3 1/400 E19 intest = nuclear anti-DPPA3 staining in small intestine of E19 mouse fetus; staining is stronger in epithelial cell nuclei towards the tip of the villus!
    (dilution of primary AB 1 : 400)
    DPPA3 contr wo 1st E19 cartl = no primary antibody, concentration of 2ndary AB as in the other pictures

    Read More

    Abcam community

    Verified customer

    Asked on Oct 01 2012

    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided; the protocol looks fine to me to however I have following further suggestions for improvements;

    - Please try Tris EDTA buffer or enzymatic antigen retrieval method; it may well be that heating tissue for 10 minutes have damaged the tissue which is why antibody is binding all over.
    - Please try blocking in 10% non immune swine serum or 5% BSA for at least 2 hours.
    - Ethanol or methanol fixation is recommended for frozen sections; the antigen retrieval method can skipped as Ethanol/methanol helps unmasking the antigen for antibody binding. Please try one section without antigen retrieval step.

    The rest of the protocol is fine. Please let me know how the staining go.

    Thank you very much for your cooperation. I will look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 Oct 01 2012

    Question

    Hi
    Some weeks ago we ordered an antibody to Stella/DPPA3 from you. Stella/
    DPPA3 has been described as marker for primordial germ cells and as
    such is not expressed in somatic cells. We applied our recently bought
    AB (GR77041-1) to sections of E19 mouse fetuses and were surprised to
    find that the AB binds to epitopes in numerous somatic cell types!

    I attach pictures that show cartilage and intestine that were exposed
    to the AB a4 1:200 and 1.400 dilutions. Secondary, HRP-labelled
    antibody was from DAKO. No unspecific staining was observed on
    sections treated only with the 2nd AB. Please note that the antibody
    labels many additional cell types in fetal tissues and the placenta!

    Treatment of paraffin sections was according to routine, i.e., antigenretrieval and peroxidase blocking were performed prior to exposure to
    1st AB.

    I wonder if some mix-up occurred, that is, that we may have received
    another AB instead of ab19878?

    I look forward to hearing from you.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    回复于 Sep 28 2012

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    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
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