Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human prostate carcinoma (PMID : 10588738).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Internal testing showed positive staining in only one case of prostate carcinoma out of seven. Because of the heterogeneous expression of STEAP1, more carcinoma cases are suggested.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human colon (PMID : 10588738).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Internal testing showed positive staining in only one case of prostate carcinoma out of seven. Because of the heterogeneous expression of STEAP1, more carcinoma cases are suggested.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) LNCaP (human prostate carcinoma epithelial cell) cell pellet. (B) PC-3 (human prostate adenocarcinoma epithelial cell) cell pellet. tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) LNCaP cell pellet no staining on (B) PC-3 cell pellet.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Internal testing showed positive staining in only one case of prostate carcinoma out of seven. Because of the heterogeneous expression of STEAP1, more carcinoma cases are suggested.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling STEAP1 with ab324263 at 1/100 (5.27 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human cerebrum (PMID : 10588738).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica BiosystemsBOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Internal testing showed positive staining in only one case of prostate carcinoma out of seven. Because of the heterogeneous expression of STEAP1, more carcinoma cases are suggested.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded HEK-293T (human embryonic kidney epithelial cell) cells labelling STEAP1 with ab324263 at 1 : 8000 (0.066 µg/ml). Positive staining on (A) HEK-293T transfected with a human STEAP1 expression vector containing a His tag no staining on (B) HEK-293T transfected with a human STEAP1B1 expression vector containing a His tag (C) HEK-293T transfected with a human STEAP1B2 expression vector containing a His tag (D) HEK-293T transfected with a human STEAP2 expression vector containing a His tag (E) HEK-293T transfected with a human STEAP3 expression vector containing a His tag (F) HEK-293T transfected with a human STEAP4 expression vector containing a His tag and (G) HEK-293T transfected with empty vector containing a His tag. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) kit.

Counterstained with Hematoxylin.

Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

Internal testing showed positive staining in only one case of prostate carcinoma out of seven. Because of the heterogeneous expression of STEAP1, more carcinoma cases are suggested.

• WB

Supplier Data

Western blot - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : PC-3 DU 145 (PMID : 37041154).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 37041154).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-STEAP1 antibody [EPR29701-75] (<a href='/products/primary-antibodies/steap1-antibody-epr29701-75-ab324263'>ab324263</a>) at 1/1000 dilution

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,32 kDa,36 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-STEAP1 antibody [EPR29701-75] - BSA and Azide free (AB325969)

This data was developed using ab324263, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human STEAP1B1 STEAP1B2 STEAP2 STEAP3 STEAP4.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-STEAP1 antibody [EPR29701-75] (<a href='/products/primary-antibodies/steap1-antibody-epr29701-75-ab324263'>ab324263</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human STEAP1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human STEAP1B1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human STEAP1B2 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human STEAP2 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human STEAP3 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a human STEAP4 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa,32 kDa,15-50 kDa,15 kDa

false

Exposure time: 6s