Anti-Staufen/STAU1抗体(ab73478)
Key features and details
- Rabbit polyclonal to Staufen/STAU1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Staufen/STAU1抗体
参阅全部 Staufen/STAU1 一抗 -
描述
兔多克隆抗体to Staufen/STAU1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human Staufen/STAU1 aa 350-450 conjugated to keyhole limpet haemocyanin.
Database link: O95793 -
阳性对照
- This antibody gave a positive signal in the following Human Whole Cell Lysates: SK N BE, SW480
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| IP | (1) |
Use at an assay dependent concentration.
|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IP
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. -
组织特异性
Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta. -
序列相似性
Contains 3 DRBM (double-stranded RNA-binding) domains. -
结构域
One of the DRDB could be involved in RER binding.
The C-terminal contains the tubulin binding domain (TBD). -
细胞定位
Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 6780 Human
- Entrez Gene: 20853 Mouse
- Entrez Gene: 84496 Rat
- Entrez Gene: 704197 Rhesus monkey
- Omim: 601716 Human
- SwissProt: O95793 Human
- SwissProt: Q9Z108 Mouse
- Unigene: 596704 Human
see all -
别名
- Double stranded RNA binding protein Staufen homolog 1 antibody
- Double stranded RNA binding protein Staufen homolog antibody
- Double-stranded RNA-binding protein Staufen homolog 1 antibody
see all
图片
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Western blot - Anti-Staufen/STAU1 antibody (ab73478)All lanes : Anti-Staufen/STAU1 antibody (ab73478) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Staufen/STAU1 antibody (ab73478)Staufen/STAU1 was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Staufen/STAU1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
Band: 63kDa: Staufen/STAU1; non specific - 75kDa: We are unsure as to the identity of this extra band. -
Immunocytochemistry/ Immunofluorescence - Anti-Staufen/STAU1 antibody (ab73478)ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Staufen/STAU1 antibody (ab73478)IHC image of Staufen/STAU1 staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab73478 被引用在 22 文献中.
- Grzejda D et al. The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity. Sci Adv 8:eabo5578 (2022). PubMed: 36170367
- Liang X et al. Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis. Adipocyte 10:350-360 (2021). PubMed: 34224297
- Lai HC et al. Regulation of RNA degradation pathways during the lipopolysaccharide response in Macrophages. J Leukoc Biol 109:593-603 (2021). PubMed: 32829531
- Bonnet-Magnaval F et al. High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation. Int J Mol Sci 23:N/A (2021). PubMed: 35008641
- Marcellus KA et al. Distinct roles for the RNA-binding protein Staufen1 in prostate cancer. BMC Cancer 21:120 (2021). PubMed: 33541283