Anti-Stathmin 1 (phospho S24)抗体[EP2124Y] (ab62336)

Thank you for confirming this information and for your help and cooperation with this case.

As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

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As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time they have spent on these experiments, it is disappointing this has not worked for the customer. I would like to offer a refund or credit note in compensation (providing the product has been purchased in the last 6 months). In order to arrange this, I would appreciate if you could confirm the order number and date of purchase?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for your message.

To confirm:

1. Growing cells were starved for 24hr in the medium without FBS.

2. Growing cells treated the with 20% FBS for 3hr.

3. Add calyculin A to the growing cells at the concentration of 100ng/mln for 30 minutes at 37oC.

4. After the 30 min incubation, the cells were harvested and lysed with RIPA buffer containing the usual protease inhibitor cocktail.

I hope this will be helpful. Should you have any further questions, please do not hesitate to contact us.

Thank you for your reply and for kindly providing this information.

Reviewing the details, I can suggest my main concern is still that the cells have not been treated.

Here is the treatment which was used in testing the cells (as shown in the WB image on the datasheet, HeLa):

1. The cells were starved for 24hr in the medium without FBS.

2. Treated the cells with 20% FBS for 3h. 3.

Added calyculin A at the concentration of 100ng/mln.

4. After 30min, the cells were harvested and lysed.

I hope this will be helpful. Please do not hesitate to let me know the results. I hope we can resolve this case as soon as possible.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab62336 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would if you can confirm some further details in order to help me investigate this further:

1. Please confirm further details of the results. Was the membrane completely clear, or was there background staining, or non specific bands at incorrect size? I would appreciate if you are able to provide an image, including molecular weight markers, which will help us to assess the results.

2. The antibody detects the phosphorylated (S24) stathmin 1. As far as I am aware, the phosphorylation can require inducing. For example, the HeLa cell lysate used in the WB shown on the datasheet has been treated with FBS+Calyculin A. Could you confirm if the cells used in these experiments have been treated? I can suggest considering a further literature search for more information.

3. Has the transfer to the membrane and quality of the sample been assessed using a loading control.

I hope this information is helpful. Please do not hesitate to contact me again with the further requested details and I hope we can resolve this case as soon as possible.