Anti-Stathmin 1抗体[EP1572] (ab119991)
![Western blot - Anti-Stathmin 1 antibody [EP1572] (ab119991)](https://www.abcam.cn/ps/products/119/ab119991/Images/ab119991-133590-anti-stathmin-1-antibody-ep1572-western-blot.jpg "Western blot - Anti-Stathmin 1 antibody [EP1572] (ab119991)")
Key features and details
- Rabbit monoclonal [EP1572] to Stathmin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
-
产品名称
Anti-Stathmin 1抗体[EP1572]
参阅全部 Stathmin 1 一抗 -
描述
兔单克隆抗体[EP1572] to Stathmin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, Human spleen, Jurkat and SH-SY5Y cell lysates; Human brain tissue. IHC-P: Human brain tissue.
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1572 -
同种型
IgG -
研究领域
相关产品
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119991于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/20000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
| 说明 |
|---|
|
WB
1/5000 - 1/20000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
靶标
-
功能
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. -
组织特异性
Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. -
序列相似性
Belongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
翻译后修饰
Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
-
数据库链接
- Entrez Gene: 3925 Human
- Entrez Gene: 16765 Mouse
- Entrez Gene: 29332 Rat
- Omim: 151442 Human
- SwissProt: P16949 Human
- SwissProt: P54227 Mouse
- SwissProt: P13668 Rat
- Unigene: 209983 Human
see all -
别名
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
图片
-
Western blot - Anti-Stathmin 1 antibody [EP1572] (ab119991)All lanes : Anti-Stathmin 1 antibody [EP1572] (ab119991) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human spleen lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (ab119991)](https://www.abcam.cn/ps/products/119/ab119991/Images/ab119991-133589-anti-stathmin-1-antibody-ep1572-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1572] (ab119991)ab119991 at 1/100 dilution staining Stathmin 1 in Paraffin-embedded Human Brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab119991 尚未被引用在任何文献中。