Anti-Stathmin 1抗体(ab131481)
Key features and details
- Rabbit polyclonal to Stathmin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Stathmin 1抗体
参阅全部 Stathmin 1 一抗 -
描述
兔多克隆抗体to Stathmin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 23-27 (
ILSPR
) of Human Stathmin 1 (UniProt P16949). -
阳性对照
- HeLa cells; 293 and HeLa cell extracts; Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. -
组织特异性
Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. -
序列相似性
Belongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
翻译后修饰
Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 3925 Human
- Omim: 151442 Human
- SwissProt: P16949 Human
- Unigene: 209983 Human
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别名
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
图片
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All lanes : Anti-Stathmin 1 antibody (ab131481) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : HeLa cell extract
Predicted band size: 17 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Stathmin 1 with ab131481 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody (ab131481)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Stathmin 1 with ab131481 at 1/50 dilution (left) or ab131481 preincubated with blocking peptide (right).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab131481 被引用在 2 文献中.
- Gao J et al. miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma. Oncol Rep 44:139-155 (2020). PubMed: 32377743
- Wang Y et al. S-adenosyl-methionine (SAM) alters the transcriptome and methylome and specifically blocks growth and invasiveness of liver cancer cells. Oncotarget 8:111866-111881 (2017). PubMed: 29340097