重组Anti-STAT6抗体[E265] (ab32108)
![Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-396573-AntiSTAT6-antibody.jpg "Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [E265] (ab32108)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E265] to STAT6
- Suitable for: WB, IP, Flow Cyt (Intra), ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-STAT6抗体[E265]
参阅全部 STAT6 一抗 -
描述
兔单克隆抗体[E265] to STAT6 -
宿主
Rabbit -
特异性
ab32108 recognises Stat-6. It does not cross react with other STAT family members -
经测试应用
适用于: WB, IP, Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human STAT6 aa 800-900 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa and Raji cells. ICC/IF: Raji cells. Flow Cyt (intra): HeLa cells IP: HeLa cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E265 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32108于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
|
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| IP |
1/50.
|
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| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa). |
|
IP
1/50. |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/250 - 1/500. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated following stimulation by IL-4 and IL-3. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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别名
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [E265] (ab32108)
Confocal image showing cytoplasmic staining in Raji cells.
Immunocytochemistry/immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labelling STAT6 with ab32108 at 10 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as a secondary antibody at 2 µg/mL. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
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![Western blot - Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-298743-anti-stat6-antibody-e265-western-blot.jpg)
Western blot - Anti-STAT6 antibody [E265] (ab32108)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-6-anti-stat6-antibody-e265-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-STAT6 antibody [E265] (ab32108)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling STAT6 with purified ab32108 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-7-anti-stat6-antibody-e265-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-STAT6 antibody [E265] (ab32108)Purified ab32108 at 1/50 dilution (2µg) immunoprecipitating STAT6 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab32108 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32108 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 100 kDa -
![Western blot - Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-481028-antistat6-antibody-e265-western-blot-human-hela-raji.JPG)
Western blot - Anti-STAT6 antibody [E265] (ab32108)All lanes : Anti-STAT6 antibody [E265] (ab32108) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 110,81 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1. 7 seconds ; Lane 2. 3 seconds
This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.
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![Anti-STAT6 antibody [E265] (ab32108)](https://www.abcam.cn/ps/products/32/ab32108/Images/ab32108-5-benefits-of-recombinant-antibodies.png)
Anti-STAT6 antibody [E265] (ab32108)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab32108 被引用在 4 文献中.
- Yan J et al. The protective effects of Phoenixin-20 in tumor necrosis factor α (TNF-α)-induced cell senescence of rheumatoid arthritis fibroblast-like synoviocytes (FLS). Aging (Albany NY) 15:14607-14616 (2023). PubMed: 38112587
- Zhang Q et al. Licochalcone E inhibits trxR1 expression, alters Nrf2/STAT6 signal, and induces antitumor effects in vitro against human SH-SY5Y and SK-N-BE(2) neuroblastoma cells. Environ Toxicol 37:1173-1184 (2022). PubMed: 35092341
- Yu L & Li J Punicalagin Alleviates Aged Bronchial Asthma by Inhibiting Th2 Differentiation through IL-4/STAT6 and Jagged1/Notch Pathways. J Healthc Eng 2022:1184677 (2022). PubMed: 35140898
- Duns G et al. Characterization of DLBCL with a PMBL gene expression signature. Blood 138:136-148 (2021). PubMed: 33684939