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AB242645

重组Anti-STAT5b抗体[EPR19487-44] - BSA and Azide free (Capture)

Anti-STAT5b antibody [EPR19487-44] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal STAT5b antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

Signal transducer and activator of transcription 5B, STAT5B

1 Images
Sandwich ELISA - Anti-STAT5b antibody [EPR19487-44] - BSA and Azide free (Capture) (AB242645)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-STAT5b antibody [EPR19487-44] - BSA and Azide free (Capture) (AB242645)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab216164).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19487-44

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody has 0.6% cross reaction with human STAT5A.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab242645 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human STAT5b. The recommended pair for sandwich ELISA is:
Capture: ab242645, Human STAT5b Capture Antibody (unconjugated)
Detector: ab242890, Human STAT5b Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STAT5b also known as Signal Transducer and Activator of Transcription 5B is a 92-kDa protein involved in signaling processes within the cell. Mechanically it functions as a transcription factor and shuttles between the cytoplasm and nucleus modulating gene expression according to extracellular signals. Upon cytokine binding STAT5b undergoes phosphorylation allowing it to form dimers and translocate to the nucleus. It is expressed in many tissues including the liver immune cells and mammary glands reflecting its role in diverse physiological processes.
Biological function summary

STAT5b plays an important role in regulating cell growth differentiation and apoptosis. It often acts as part of the STAT protein family binding to specific DNA sequences to activate transcription. STAT5b particularly influences immune function and hematopoiesis driving proliferation and differentiation of lymphoid and myeloid cells. Its activity affects glucose homeostasis growth hormone signaling and lactation highlighting its importance in various physiological states and developmental processes.

Pathways

STAT5b integrates into the JAK-STAT signaling pathway a critical route for conveying extracellular information to the nucleus. This pathway includes JAK kinases which activate STAT5b by phosphorylation upon cytokine-receptor engagement. STAT5b also participates in the growth hormone pathway interacting with proteins such as Growth Hormone Receptor and other STAT family members like STAT3. Through these interactions STAT5b helps regulate gene expression associated with growth and differentiation in response to hormonal signals.

STAT5b links to several conditions such as growth hormone insensitivity and leukemia. In growth disorders defects in STAT5b can impair normal growth hormone signal transduction often involving interplay with proteins like the Growth Hormone Receptor. In leukemias aberrant activation or expression of STAT5b contributes to uncontrolled cellular proliferation often accompanied by deregulation of the JAK-STAT pathway. Research into STAT5b offers insights into potential therapeutic targets for these and other related conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Carries out a dual function : signal transduction and activation of transcription (PubMed : 29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
See full target information STAT5B
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