Anti-STAT5a (phospho Y694)抗体(ab30648)
Key features and details
- Rabbit polyclonal to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-STAT5a (phospho Y694)抗体
参阅全部 STAT5a 一抗 -
描述
兔多克隆抗体to STAT5a (phospho Y694) -
宿主
Rabbit -
特异性
ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human STAT5a (phospho Y694).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30648于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6776 Human
- Omim: 601511 Human
- SwissProt: P42229 Human
- Unigene: 437058 Human
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别名
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody (ab30648)Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
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Western blot - Anti-STAT5a (phospho Y694) antibody (ab30648)All lanes : Anti-STAT5a (phospho Y694) antibody (ab30648)
Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with EGF
Predicted band size: 91 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab30648 被引用在 9 文献中.
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Jiang Y et al. Loss of STAT5A promotes glucose metabolism and tumor growth through miRNA-23a-AKT signaling in hepatocellular carcinoma. Mol Oncol 15:710-724 (2021). PubMed: 33155364
- Kaihoko Y et al. Distinct expression patterns of aquaporin 3 and 5 in ductal and alveolar epithelial cells in mouse mammary glands before and after parturition. Cell Tissue Res 380:513-526 (2020). PubMed: 31953689
- Wang S et al. Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. J Hematol Oncol 11:82 (2018). PubMed: 29898759
- Matsunaga K et al. IL-1ß directly inhibits milk lipid production in lactating mammary epithelial cells concurrently with enlargement of cytoplasmic lipid droplets. Exp Cell Res 370:365-372 (2018). PubMed: 29966663
- Kobayashi K et al. Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ß-casein expression in mammary epithelial cells. Biochim Biophys Acta 1863:2006-16 (2016). PubMed: 27130254
- Mace TA et al. Single agent BMS-911543 Jak2 inhibitor has distinct inhibitory effects on STAT5 signaling in genetically engineered mice with pancreatic cancer. Oncotarget 6:44509-22 (2015). IHC . PubMed: 26575024
- Surana R et al. IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model. Cancer Immunol Res 2:1103-12 (2014). PubMed: 25204776
- Bertucci PY et al. Glucocorticoid-induced impairment of mammary gland involution is associated with STAT5 and STAT3 signaling modulation. Endocrinology 151:5730-40 (2010). PubMed: 20881248