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AB264324

Anti-STAT5a抗体

Anti-STAT5a antibody

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Rabbit Polyclonal STAT5a antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human STAT5A aa 700 to C-terminus.

查看别名

STAT5, STAT5A, Signal transducer and activator of transcription 5A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody (AB264324)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody (AB264324)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling STAT5a with ab264324 at 1/200 dilution. Detection : DAB.

Immunoprecipitation - Anti-STAT5a antibody (AB264324)
  • IP

Supplier Data

Immunoprecipitation - Anti-STAT5a antibody (AB264324)

STAT5a was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264324 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264324 at 1 μg/ml.
Lane 1 : ab264324 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

ab264324 is specific for STAT5a.

All lanes:

Immunoprecipitation - Anti-STAT5a antibody (ab264324)

Predicted band size: 90 kDa

false

Western blot - Anti-STAT5a antibody (AB264324)
  • WB

Supplier Data

Western blot - Anti-STAT5a antibody (AB264324)

All lanes:

Western blot - Anti-STAT5a antibody (ab264324) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 90 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, IP, WB

applications

免疫原

Synthetic Peptide within Human STAT5A aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42229

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替代产品

Primary Antibodies

AB194309

Alexa Fluor® 647 Anti-STAT5a antibody [E289]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-STAT5a antibody [E289] (AB194309)

  • 0
  • 0
Primary Antibodies

AB128896

Anti-STAT5a (phospho S726) antibody [EPR1914(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] (AB128896)

  • 0
  • 0
Primary Antibodies

AB32043

Anti-STAT5a antibody [E289]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] (AB32043)

  • 5
  • 2
Primary Antibodies

AB248206

Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-STAT5a (phospho S726) antibody [EPR1914(2)] - BSA and Azide free (AB248206)

  • 0
  • 0
Primary Antibodies

AB213219

Anti-STAT5a antibody [E289] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a antibody [E289] - BSA and Azide free (AB213219)

  • 0
  • 0

搭配使用产品

Assay Kits

AB205714

STAT 5 A/B ELISA Kit

SimpleStep

Sandwich ELISA - STAT 5 A/B ELISA Kit (AB205714)

  • 5
  • 1
Primary Antibodies

AB106095

Anti-STAT5a (phospho Y694) antibody [6.F1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody [6.F1] (AB106095)

  • 4
  • 1
Primary Antibodies

AB30649

Anti-STAT5a (phospho S780) antibody

Immunocytochemistry/ Immunofluorescence - Anti-STAT5a (phospho S780) antibody (AB30649)

  • 0
  • 0
Primary Antibodies

AB178941

Anti-STAT5b antibody [EPR16671]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (AB178941)

  • 5
  • 4
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STAT5a also known as Signal Transducer and Activator of Transcription 5A acts as a transcription factor in the cell. STAT5a weighs approximately 90 kDa and expresses in various tissues including the liver muscle and hematopoietic cells. It plays a central role in mediating cellular responses to cytokines and growth factors particularly those in the interleukin-2 (IL-2) family and growth hormone (GH).
Biological function summary

Many systems use STAT5a to regulate cell proliferation differentiation and survival. It often forms a complex with other proteins like STAT5b which is a closely related counterpart and both contribute to similar cellular processes. STAT5a can dimerize upon activation allowing it to move to the cell nucleus to influence gene expression directly.

Pathways

Researchers place STAT5a prominently in the JAK-STAT signaling pathway. This pathway is essential for transmitting information received from extracellular chemical signals to the cell nucleus resulting in the activation of genes controlling cellular functions. STAT5a works together with Janus kinases (JAKs) to mediate various biological responses including hormone signaling and immune function. It also interacts with proteins like IL-2 receptor subunits which are involved in many cytokine-driven processes.

Disturbances in STAT5a activity link specifically with conditions like leukemia and breast cancer. Overactivation or mutation can lead to uncontrolled cell growth and survival contributing to these diseases. In leukemia STAT5a frequently partners with other proteins like BCR-ABL which is known for its role in chronic myeloid leukemia influencing oncogenic pathways. In breast cancer alterations in STAT5a signaling may interact with estrogen and progesterone receptors affecting tumor progression and response to therapies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A
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