AB278764

重组Anti-STAT5 (phospho Y694)抗体[Stat5Y694-G11]

Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]

20ul selling size
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal STAT5a phospho Y694 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human STAT5A pY694.

查看别名

STAT5, STAT5A, Signal transducer and activator of transcription 5A

5 Images

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 μg/mL isotype control or untreated (red) or pervanadate (green) using ab278764.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

Flow cytometric analysis of 3T3 cells secondary antibody only negative control (blue) or untreated (grey) or treated with pervanadate (orange) using 0.1 μg/mL isotype control or untreated (red) or pervanadate (green) using ab278764 at 0.1 μg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

Peptide blocking flow cytometric analysis of 3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or pervanadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278764 at 0.1 μg/mL.

Supplier Data

Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

All lanes:

Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/mL

Lane 1:

K562 cell extract untreated

Lane 2:

K562 cell extract treated with imatinib

Predicted band size: 91 kDa

false

Supplier Data

Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

Lanes 1 - 2:

Western blot - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (ab278764) at 0.01 µg/mL

Lanes 3 - 4:

No primary antibody control

Lanes 1 and 3:

K562 cell extract untreated

Lanes 2 and 4:

K562 cell extract treated with imatinib

Predicted band size: 91 kDa

false

不同偶联物与剂型 (3)

660 APC

APC Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]
519 FITC

FITC Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]
578 PE

PE Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Stat5Y694-G11

亚型

IgG

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Human

应用

Flow Cyt, WB

applications

免疫原

Synthetic Peptide within Human STAT5A pY694. The exact immunogen used to generate this antibody is proprietary information.

P42229

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-1 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001-1 µg/mL", "WB-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.

Biological function summary

STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.

Pathways

STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.

STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

See full target information STAT5A pY694

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251261 PubMed40918151

2025

CircASH1L-mediated tumor progression in triple-negative breast cancer: PI3K/AKT pathway mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Liu,Jiaming Wu,Jin Gong,Jing Zhu,Jie Liu,Xiaoqing Chen,Shaohua Qu,Jintao Chen,Zhisheng Zhou,Xulong Fan

Journal of cellular and molecular medicine 29:e70421 PubMed39972942

2025

LncRNA FAM30A Suppresses Proliferation and Metastasis of Colorectal Carcinoma by Blocking the JAK-STAT Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Liu,Shuangyin Han,Yuanbo Cui,Qiuyan Zhao,Yunfei Wang,Tian Li,Xiuling Li

Respiratory research 25:428 PubMed39633343

2024

T follicular helper cell is essential for M2 macrophage polarization and pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Li,Pingping Liu,Hao Zhu,Huan Yang,Jun Zha,Huiling Yao,Shaoze Zhang,Jin Huang,Guang Li,Gang Jiang,Yongliang Jiang,Aiguo Dai

Aging 16:12498-12509 PubMed38747738

2024

SHP2 mediates STAT3/STAT6 signaling pathway in TAM to inhibit proliferation and metastasis of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guojing Chai,Yingbo Nan,Haili Zhao,Qingchuan Hu

Journal of inflammation research 15:1797-1810 PubMed35300210

2022

Mannan-Binding Lectin Regulates the Th17/Treg Axis Through JAK/STAT and TGF-β/SMAD Signaling Against Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Fanping Wang,Yonghui Yang,Zhixin Li,Yan Wang,Zhenchao Zhang,Wei Zhang,Yonghui Mu,Jingwen Yang,Lili Yu,Mingyong Wang

View all publications

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重组Anti-STAT5 (phospho Y694)抗体[Stat5Y694-G11]