重组Anti-STAT5 (phospho Y694)抗体[E208] (ab32364)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E208] to STAT5 (phospho Y694)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-STAT5 (phospho Y694)抗体[E208]
参阅全部 STAT5 一抗 -
描述
兔单克隆抗体[E208] to STAT5 (phospho Y694) -
宿主
Rabbit -
特异性
This reagent is not recommended for mouse IHC-P
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经测试应用
适用于: WB, IHC-P, ELISAmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E208 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32364于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Unsuitable for mouse. |
ELISA |
Use at an assay dependent concentration.
|
说明 |
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WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Unsuitable for mouse. |
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
see all -
别名
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
图片
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All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (purified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Epidermal growth factor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
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All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (unpurified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lane 3 : A431 cell lysate, treated with phosphatase
Predicted band size: 90 kDa
Observed band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (70)
ab32364 被引用在 70 文献中.
- Li Y et al. CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice. Pathogens 12:N/A (2023). PubMed: 38003764
- Yang C et al. Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment. Cell Death Dis 14:525 (2023). PubMed: 37582769
- Li J et al. Targeting of STAT5 using the small molecule topotecan hydrochloride suppresses acute myeloid leukemia progression. Oncol Rep 50:N/A (2023). PubMed: 37830151
- Cao L et al. CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response. Cell Transplant 32:9636897221149444 (2023). PubMed: 36644879
- Vaparanta K et al. STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth. EMBO Rep 24:e56689 (2023). PubMed: 37009825