Anti-STAT2 (phospho Y690)抗体(ab53132)
Key features and details
- Rabbit polyclonal to STAT2 (phospho Y690)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-STAT2 (phospho Y690)抗体
参阅全部 STAT2 一抗 -
描述
兔多克隆抗体to STAT2 (phospho Y690) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human STAT2 aa 650-750 (phospho Y690). Around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).
Database link: P52630 -
阳性对照
- IHC-P: Rat kidney, Human colon. WB: HeLa cells treated with IFN.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53132于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IFN-alpha. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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数据库链接
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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别名
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
图片
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Western blot - Anti-STAT2 (phospho Y690) antibody (ab53132)All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution
Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide
Predicted band size: 98 kDa
Observed band size: 98 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 (phospho Y690) antibody (ab53132)ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab53132 被引用在 25 文献中.
- Ji W et al. TP53TG1/STAT axis is involved in the development of colon cancer through affecting PD-L1 expression and immune escape mechanism of tumor cells. Am J Cancer Res 13:5218-5235 (2023). PubMed: 38058799
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Liang Y et al. Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis. Exp Ther Med 22:875 (2021). PubMed: 34194553
- Wong SW et al. Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget's disease. Cell Death Differ 27:71-84 (2020). PubMed: 31076632
- LaClair KD et al. Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS. Acta Neuropathol 140:121-142 (2020). PubMed: 32562018