AB109461

重组Anti-STAT1 (phospho S727)抗体[EPR3146]

Name: STAT1(磷酸化S727)抗体[EPR3146] (ab109461)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109461
Price: 1604 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-STAT1 (phospho S727) antibody [EPR3146]

20ul selling size
Recombinant
Advanced Validation
RabMAb
了解详情

(1 Review)

(92 Publications)

Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) is a rabbit monoclonal antibody detecting STAT1 in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

查看别名

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Unpurified ab109461, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Immunohistochemical staining of paraffin embedded mouse colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Immunohistochemical staining of paraffin embedded rat colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Lab

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Blocking buffer : 2% BSA/TBST
Dilution buffer : 2% BSA/TBST

All lanes:

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/5000 dilution

Lane 1:

Untreated HeLa whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate treated with etoposide at 10 µg

Lane 3:

HeLa whole cell lysate treated with etoposide, followed by membrane treatment with phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab181602 was used as a GAPDH loading at 1000000 dilution.

All lanes:

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution

Lane 1:

Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 treated with 50 ng/ml IFN beta for 3 hours whole cell lysate at 20 µg

Lane 3:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 treated with 100 ng/ml IFN alpha for 24 hours whole cell lysate at 20 µg

Lane 5:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 treated with 100 ng/ml IFN gamma for 48 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 91 kDa,37 kDa

Observed band size: 91 kDa,37 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

The lower section shows STAT1 detected with ab92506, anti-STAT1 antibody, to confirm that the same amount of lysate is used in each lane.

Blocking and dilution biffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/10000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma) at 10 µg

Lane 2:

HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution

Predicted band size: 87 kDa

Observed band size: 91 kDa

true

Exposure time: 30s

ChIC/CUT&RUN sequencing - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) treated with IFN gamma (50ng/ml 1h) cells and 5µg of ab109461 [EPR3146]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Lab

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution

Lane 1:

Rat brain lysate at 15 µg

Lane 2:

Rat brain lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 91 kDa

false

Lab

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg

Lane 3:

Mouse brain lysate at 15 µg

Lane 4:

Mouse brain lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 91 kDa

false

Lab

Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)

Dot Blot analysis of Lane 1 : STAT1 (pS727) phospho peptide and Lane 2 : STAT1 non-phospho peptide, labeling STAT1 (phospho S727) with ab109461 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time : 3 minutes.

不同偶联物与剂型 (1)

Carrier free

Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3146

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, Dot, ChIC/CUT&RUN-seq, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

A phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "5 µg", "ChICCUTRUNseq-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." }, "Rat": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes." } } }

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产品详情

What is this antibody validated in?
Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of STAT1?
Anti-STAT1 (phospho S727) [EPR3146] (ab109461) specifically detects a band for STAT1 (UniProt: P42224) at a molecular weight of 87kDa.

Trusted by the scientific community
Anti-STAT1 (phospho S727) [EPR3146] (ab109461) was first used in a scientific publication in 2011 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3146] also available for your convenience: ab109461, Carrier free - ab215820

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

See full target information STAT1 phospho S727

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文献 (92)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed41011190

2025

Nagilactone C from the Seeds of May Protect Against LPS-Induced Acute Lung Injury via STAT Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Chen,Jing Tang,Shijie Zhan,Yixian Qiu,Jing Li,Weiguang Shan,Youmin Ying

Frontiers in pharmacology 16:1646545 PubMed40860883

2025

Zuoqing granules attenuate ulcerative colitis via macrophage polarization modulation: involvement of the PPAR-γ/NF-κB/STAT1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Deng,Ming Li,Xiaoli Fang,Kun Tang,Shuqing Xu,Rui Ding,Zilong Li

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

International journal of molecular sciences 26: PubMed40332339

2025

IRF5 Mediates Artery Inflammation in Salt-Sensitive Hypertension by Regulating STAT1 and STAT2 Phosphorylation to Increase ESM1 Transcription: Insights from Bioinformatics and Mechanistic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyu Shao,Hao Wang,Shicheng Li,Mengying Zeng,Shuyang Zhang,Xiaowei Yan

CNS neuroscience & therapeutics 31:e70404 PubMed40277259

2025

Balancing Anti-Inflammation and Neurorepair: The Role of Mineralocorticoid Receptor in Regulating Microglial Phenotype Switching After Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhang,Miao Bai,Mengshi Yang,Yumei Wang,Xueling Zhang,Xiyu Chen,Min Gao,Baiyun Liu,Guangzhi Shi

Molecular cancer 24:34 PubMed39876004

2025

Sorafenib enhanced the function of myeloid-derived suppressor cells in hepatocellular carcinoma by facilitating PPARα-mediated fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Li,Liting Xiong,Yuhan Yang,Ping Jiang,Junjie Wang,Mengyuan Li,Shuhua Wei,Suqing Tian,Yuexuan Wang,Mi Zhang,Jie Tang

International journal of molecular sciences 26: PubMed39796271

2025

Single and Combined Impact of Semaglutide, Tirzepatide, and Metformin on β-Cell Maintenance and Function Under High-Glucose-High-Lipid Conditions: A Comparative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Esmaeel Ghasemi Gojani,Bo Wang,Dongping Li,Olga Kovalchuk,Igor Kovalchuk

Frontiers in immunology 15:1472425 PubMed39660129

2024

Repression of JAK2-STAT1 and PD-L1 by CEP-33779 ameliorates the LPS-induced decline in phagocytic activity of alveolar macrophages and mitigates lung injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han Wang,A-Guo Li,Hong-Yan Wang,Yong-Sheng Tu

PeerJ 12:e18612 PubMed39619202

2024

The effects of plasma from patients with active thyroid-associated orbitopathy on the survival and inflammation of melanoma-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Huifang Chen,Shiyuan Chen,Zhenfeng Liu

PeerJ 12:e18507 PubMed39559329

2024

Cirsilineol improves anesthesia/surgery-induced postoperative cognitive dysfunction through attenuating oxidative stress and modulating microglia M1/M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Junli Du,Chao Chen,Jie Chen

View all publications

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chicCutRunSequencingBooklet

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