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AB3987

Anti-STAT1 抗体 [SM1]

Anti-STAT1 antibody [SM1]

3

(3 Reviews)

|

(26 Publications)

Mouse Monoclonal STAT1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human STAT1 aa 700 to C-terminus.

查看别名

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

3 Images
Flow Cytometry - Anti-STAT1 antibody [SM1] (AB3987)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-STAT1 antibody [SM1] (AB3987)

Overlay histogram showing HeLa cells stained with ab3987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3987, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-STAT1 antibody [SM1] (AB3987)
  • WB

Supplier Data

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Western blotting analysis of STAT1 using ab3987 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions.

Nitrocellulose membrane was probed with 2 μg/ml of ab3987 followed by IRDye800-conjugated anti-mouse secondary antibody.

All lanes:

Western blot - Anti-STAT1 antibody [SM1] (ab3987) at 2 µg/mL

Lane 1:

HeLa cell lysate (reducing conditions)

Lane 2:

MCF-7 cell lysate (reducing conditions)

Lane 3:

Cos-7 cell lysate (reducing conditions)

Secondary

All lanes:

IRDye800-conjugated anti-mouse

Predicted band size: 87 kDa

Observed band size: 90 kDa

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Western blot - Anti-STAT1 antibody [SM1] (AB3987)
  • WB

Unknown

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727) by the antibody PSM1 (upper panel) and total STAT 1 level by the antibody SM1 (lower panel).

All lanes:

Western blot - Anti-STAT1 antibody [SM1] (ab3987)

Predicted band size: 87 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

SM1

亚型

IgG2b

不含载体蛋白

No

反应种属

Human

应用

WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human STAT1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42224

特异性

The antibody recognizes an epitope included within amino acids 721-733 of the 91 kDa STAT 1 protein.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.
Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).
See full target information STAT1
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文献 (26)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:1114 PubMed37968576

2023

ESCO2 promotes hypopharyngeal carcinoma progression in a STAT1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hu,Jing Yan,Yijie Chen,Xiaohui Li,Liu Yang,Haiyu Di,Huihui Zhang,Yewen Shi,Junjie Zhao,Yanxia Shi,Yinglong Xu,Xiaoyong Ren,Zhenghui Wang

American journal of cancer research 13:5218-5235 PubMed38058799

2023

TP53TG1/STAT axis is involved in the development of colon cancer through affecting PD-L1 expression and immune escape mechanism of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenmin Ji,Wenyan Wang,Yanfang Wei

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 24:927-940 PubMed35107757

2022

STAT1/IFIT2 signaling pathway is involved in PD-L1-mediated epithelial-to-mesenchymal transition in human esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J Chen,Y Liu,Y Zhu,Y Chen,J Feng,T Jiang,X Zheng,L Chen,J Jiang

Open medicine (Warsaw, Poland) 16:1642-1652 PubMed34761115

2021

miR-150-5p affects AS plaque with ASMC proliferation and migration by STAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Bian,Wenqiang Cai,Hongying Lu,Shuhong Tang,Keqin Yang,Yan Tan

Life sciences 277:119419 PubMed33785336

2021

LncRNA DLEU2 is activated by STAT1 and induces gastric cancer development via targeting miR-23b-3p/NOTCH2 axis and Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchun Li,Zhen Zhang,Zhaosheng Chen,Bin Liu,Honglei Wu

American journal of translational research 13:210-222 PubMed33527019

2021

Schisandrin C attenuates renal damage in diabetic nephropathy by regulating macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jingqiu Cui,Ming Liu,Yingqi Shao,Xiaoying Dong

Cell death & disease 11:693 PubMed32826878

2020

TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Xu,Jin Wang,Jianyuan Jiang,Jian Song,Wei Zhu,Fan Zhang,Minghao Shao,Haocheng Xu,Xiaosheng Ma,Feizhou Lyu

Genome biology 21:140 PubMed32539747

2020

Dynamic rewiring of the human interactome by interferon signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Craig H Kerr,Michael A Skinnider,Daniel D T Andrews,Angel M Madero,Queenie W T Chan,R Greg Stacey,Nikolay Stoynov,Eric Jan,Leonard J Foster

Journal of neuroinflammation 17:134 PubMed32345320

2020

LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Zhou,Zhiyue Li,Tianding Wu,Qun Zhao,Qiancheng Zhao,Yong Cao

Oncology letters 20:248-256 PubMed32565951

2020

STAT1 is a modulator of the expression of frequently rearranged in advanced T-cell lymphomas 1 expression in U251 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Guo,Shule Wang,Yining Hao,Yeqing Ren,Yongqiang Wu,Jianping Zhang,Dong Liu
View all publications
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