AB200344

重组Anti-STARD4抗体[EPR17847]

Anti-STARD4 antibody [EPR17847]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal STARD4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

StAR-related lipid transfer protein 4, START domain-containing protein 4, StARD4, STARD4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (AB200344)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847] (AB200344)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STARD4 with ab200344 at a 1/100 dilution. ab150077 AlexaFluor®488 Goat anti-Rabbit secondary 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) 1/1000 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary 1/500 dilution. Cells were fixed in 100% methanol and permeabilized using 0.1% Triton X-100. Nuclear stain DAPI.

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847] (AB200344)

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

U-937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-STARD4 antibody [EPR17847] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17847

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STARD4 or StAR-related lipid transfer protein 4 is a cytosolic protein involved in cholesterol transport. It is approximately 22 kDa in size. This protein facilitates the shuttling of cholesterol between cellular membranes and the cytoplasm. Expression of STARD4 is found in various tissues but it is notably present in the liver and macrophages. Its role in these tissues supports overall cholesterol homeostasis by regulating intracellular cholesterol distribution.

Biological function summary

This protein participates in intracellular lipid trafficking as part of larger biosynthetic and regulatory processes. It does not function independently but rather interacts with several lipid-binding proteins to control cholesterol availability within the cell. These interactions are essential for maintaining membrane integrity and the synthesis of steroid hormones. The activity of STARD4 directly influences the regulation of lipid metabolism and energy homeostasis.

Pathways

STARD4 contributes to lipid metabolism and homeostasis pathways. It plays a role in the cholesterol transport pathway specifically in concert with proteins like STARD1 and STARD5 which also bind and transfer cholesterol. Its function ensures proper cholesterol distribution required for cellular signaling and membrane structure. The activity of STARD4 intersects with other proteins involved in these pathways such as apolipoproteins and other lipid-binding proteins to maintain lipid balance within tissues particularly in organs like the liver.

STARD4 is associated with conditions linked to cholesterol imbalance such as atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Altered expression or function of STARD4 can disrupt normal cholesterol homeostasis contributing to the development of these disorders. Research indicates that its interactions with proteins such as low-density lipoprotein (LDL) receptors and apolipoprotein E (ApoE) are critical in understanding disease mechanisms. Modification in the function or expression of STARD4 can signify altered lipid transport and contribute to pathogenesis in related disorders.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Involved in the intracellular transport of cholesterol. Binds cholesterol or other sterols.

See full target information StAR-related lipid transfer protein 4

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 42:319 PubMed38008713

2023

Hsa_circ_0009092/miR-665/NLK signaling axis suppresses colorectal cancer progression via recruiting TAMs in the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Song,Qing Liu,Lei Han,Tiantian Song,Sihao Huang,Xinyao Zhang,Qiuming He,Chenxi Liang,Shuai Zhu,Bin Xiong

iScience 26:106559 PubMed37123228

2023

Multiomic landscape of immune pathogenesis in Kimura's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xunyao Wu,Anqi Wang,Sumei Zhang,Xiaoxi Wang,Dan Guo,Weiguo Zhu,Yang Jiao,Jiaxin Zhou,Wen Zhang,Linyi Peng,Minghui Duan,Yunyun Fei

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-STARD4抗体[EPR17847]