AB202060

重组Anti-STARD4抗体[EPR17847-32]

Anti-STARD4 antibody [EPR17847-32]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal STARD4 antibody. Suitable for IP, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

查看别名

StAR-related lipid transfer protein 4, START domain-containing protein 4, StARD4, STARD4

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847-32] (AB202060)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-STARD4 antibody [EPR17847-32] (AB202060)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STRAD4 with ab202060 at 1/1000 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202060 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Supplier Data

Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] (AB202060)

STARD4 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate with ab202060 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab202060 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input). Lane 2 : ab202060 IP in NIH/3T3 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202060 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-STARD4 antibody [EPR17847-32] (ab202060)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] (AB202060)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

U937 (Human histiocytic lymphoma cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] (AB202060)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] (AB202060)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Lane 5:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-STARD4 antibody [EPR17847-32] (AB202060)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-STARD4 antibody [EPR17847-32] (ab202060) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse spleen tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

不同偶联物与剂型 (1)

Carrier free

Anti-STARD4 antibody [EPR17847-32] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17847-32

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

STARD4 or StAR-related lipid transfer protein 4 is a cytosolic protein involved in cholesterol transport. It is approximately 22 kDa in size. This protein facilitates the shuttling of cholesterol between cellular membranes and the cytoplasm. Expression of STARD4 is found in various tissues but it is notably present in the liver and macrophages. Its role in these tissues supports overall cholesterol homeostasis by regulating intracellular cholesterol distribution.

Biological function summary

This protein participates in intracellular lipid trafficking as part of larger biosynthetic and regulatory processes. It does not function independently but rather interacts with several lipid-binding proteins to control cholesterol availability within the cell. These interactions are essential for maintaining membrane integrity and the synthesis of steroid hormones. The activity of STARD4 directly influences the regulation of lipid metabolism and energy homeostasis.

Pathways

STARD4 contributes to lipid metabolism and homeostasis pathways. It plays a role in the cholesterol transport pathway specifically in concert with proteins like STARD1 and STARD5 which also bind and transfer cholesterol. Its function ensures proper cholesterol distribution required for cellular signaling and membrane structure. The activity of STARD4 intersects with other proteins involved in these pathways such as apolipoproteins and other lipid-binding proteins to maintain lipid balance within tissues particularly in organs like the liver.

STARD4 is associated with conditions linked to cholesterol imbalance such as atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Altered expression or function of STARD4 can disrupt normal cholesterol homeostasis contributing to the development of these disorders. Research indicates that its interactions with proteins such as low-density lipoprotein (LDL) receptors and apolipoprotein E (ApoE) are critical in understanding disease mechanisms. Modification in the function or expression of STARD4 can signify altered lipid transport and contribute to pathogenesis in related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the intracellular transport of cholesterol. Binds cholesterol or other sterols.

See full target information StAR-related lipid transfer protein 4

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101556 PubMed40831538

2025

Targeting STARD4/EGFR axis inhibits growth and overcomes lenvatinib resistance in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengting Liu,Yixin Liu,Jiahui Zheng,Xiangping An,Jiayong Wen,Fengchi Zhu,Jin Jia,Dan Guo,Nana Chen

Nature communications 13:1135 PubMed35241650

2022

Intestinal AMPK modulation of microbiota mediates crosstalk with brown fat to control thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eryun Zhang,Lihua Jin,Yangmeng Wang,Jui Tu,Ruirong Zheng,Lili Ding,Zhipeng Fang,Mingjie Fan,Ismail Al-Abdullah,Rama Natarajan,Ke Ma,Zhengtao Wang,Arthur D Riggs,Sarah C Shuck,Li Yang,Wendong Huang

Clinical and translational medicine 11:e449 PubMed34185414

2021

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhou,Cai Zhang,Xiong Yang,Lilong Liu,Junyi Hu,Yaxin Hou,Hong Tao,Haruhiko Sugimura,Zhaohui Chen,Liang Wang,Ke Chen

Oncology reports 44:2487-2502 PubMed33125124

2020

STARD4 promotes breast cancer cell malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Zhen Xiang,Feng Wang,Rong Shan,Ling Li,Juan Chen,Bao-An Liu,Juan Huang,Lun-Quan Sun,Wei-Bing Zhou

Biochimica et biophysica acta. Molecular and cell 1865:158609 PubMed31917335

2020

Stable reduction of STARD4 alters cholesterol regulation and lipid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

David B Iaea,Zachary R Spahr,Rajesh K Singh,Robin B Chan,Bowen Zhou,Rohan Bareja,Olivier Elemento,Gilbert Di Paolo,Xiaoxue Zhang,Frederick R Maxfield

Molecular biology of the cell 28:1111-1122 PubMed28209730

2017

Role of STARD4 in sterol transport between the endocytic recycling compartment and the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

David B Iaea,Shu Mao,Frederik W Lund,Frederick R Maxfield

View all publications

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重组Anti-STARD4抗体[EPR17847-32]