重组Anti-StAR抗体[EP7639] (ab133657)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7639] to StAR
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-StAR抗体[EP7639]
参阅全部 StAR 一抗 -
描述
兔单克隆抗体[EP7639] to StAR -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human StAR aa 250 to the C-terminus. The exact sequence is proprietary.
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阳性对照
- Human adrenal gland lysate; Human adrenal gland tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP7639 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133657于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. -
组织特异性
Expressed in gonads, adrenal cortex and kidney. -
通路
Steroid metabolism; cholesterol metabolism. -
疾病相关
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted. -
序列相似性
Contains 1 START domain. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- Unigene: 521535 Human
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别名
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
see all
图片
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Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
ab133657 showing positive staining in Normal testis tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
ab133657 showing positive staining in Adrenocortical carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
ab133657 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)
ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab133657 被引用在 11 文献中.
- Källsten L et al. Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies. Cells 11:N/A (2022). PubMed: 36230992
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
- Ma J et al. Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity. Cell Biosci 11:125 (2021). PubMed: 34233737
- Bose HS et al. Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB). Mol Cell Biol 41:e0035721 (2021). PubMed: 34460330
- Asiabi P et al. Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†. Biol Reprod 102:145-155 (2020). PubMed: 31504196