Anti-ST7抗体(ab122459)
Key features and details
- Rabbit polyclonal to ST7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ST7抗体 -
描述
兔多克隆抗体to ST7 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: PQARISAAHE ALEINEIRSR VEVPLIASST IWEIKLLPKC ATAY, corresponding to amino acids of Human ST7.
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阳性对照
- RT 4 and U 251 cell line lysates; Human heart muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May act as a tumor suppressor. -
组织特异性
Ubiquitously expressed, with highest levels in heart, liver and pancreas. -
序列相似性
Belongs to the ST7 family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7982 Human
- Omim: 600833 Human
- SwissProt: Q9NRC1 Human
- Unigene: 368131 Human
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别名
- ETS7q antibody
- FAM4A antibody
- FAM4A1 antibody
see all
图片
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Western blot - Anti-ST7 antibody (ab122459)All lanes : Anti-ST7 antibody (ab122459) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Immunocytochemistry/ Immunofluorescence - Anti-ST7 antibody (ab122459)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122459 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST7 antibody (ab122459)ab122459, at 1/500, staining ST7 in paraffin embedded Human heart muscle tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST7 antibody (ab122459)Immunohistochemical staining of Human pancreas shows moderate membranous positivity using ab122459
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122459 被引用在 1 文献中.
- Zhang B et al. MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation. Mol Oncol 15:3062-3075 (2021). PubMed: 33793053