重组Anti-ST3GAL3抗体[EPR10006(2)] (ab177959)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-205996-anti-st3gal3-antibody-epr100062-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10006(2)] to ST3GAL3
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ST3GAL3抗体[EPR10006(2)]
参阅全部 ST3GAL3 一抗 -
描述
兔单克隆抗体[EPR10006(2)] to ST3GAL3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human ST3GAL3 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q11203 -
阳性对照
- Fetal heart and human skeletal muscle lysates; fetal cardiac muscle tissue; Human skeletal muscle tissue; A673 cells; permealized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10006(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177959于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
|
ICC/IF
1/50 - 1/100. |
靶标
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功能
Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc. -
组织特异性
Highly expressed in adult skeletal muscle and in all fetal tissues examined and to a much lesser extent in placenta, lung and liver. -
通路
Protein modification; protein glycosylation. -
疾病相关
Mental retardation, autosomal recessive 12
Epileptic encephalopathy, early infantile, 15 -
序列相似性
Belongs to the glycosyltransferase 29 family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing. -
细胞定位
Golgi apparatus, Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. - Information by UniProt
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数据库链接
- Entrez Gene: 6487 Human
- Entrez Gene: 20441 Mouse
- Entrez Gene: 64445 Rat
- Omim: 606494 Human
- SwissProt: Q11203 Human
- SwissProt: P97325 Mouse
- SwissProt: Q02734 Rat
- Unigene: 597915 Human
see all -
别名
- 3 sialyltransferase antibody
- 3(4) GlcNAc alpha-2 antibody
- 3-sialyltransferase 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)
Immunohistochemical analysis of Human skeletal muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-205998-anti-st3gal3-antibody-epr100062-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling ST3GAL3 using ab177959 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-205997-anti-st3gal3-antibody-epr100062-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)Immunofluorescence analysis of A673 cells labeling ST3GAL3 using ab177959 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-205995-anti-st3gal3-antibody-epr100062-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)Immunohistochemical analysis of fetal cardiac muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-205994-anti-st3gal3-antibody-epr100062-western-blot.jpg)
Western blot - Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)All lanes : Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa -
![Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)](https://www.abcam.cn/ps/products/177/ab177959/Images/ab177959-6-benefits-of-recombinant-antibodies.png)
Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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