Anti-ST14抗体- Catalytic domain (ab28266)
Key features and details
- Rabbit polyclonal to ST14 - Catalytic domain
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ST14抗体- Catalytic domain
参阅全部 ST14 一抗 -
描述
兔多克隆抗体to ST14 - Catalytic domain -
宿主
Rabbit -
特异性
This antibody does not recognize matriptase 2 (MTSP2). -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide based on the catalytic domain of Human ST14.
参阅Abcam的专有抗源政策 (Peptide available as ab41264.) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28266于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. -
疾病相关
Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 4 LDL-receptor class A domains.
Contains 1 peptidase S1 domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6768 Human
- Omim: 606797 Human
- SwissProt: Q9Y5Y6 Human
- Unigene: 504315 Human
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别名
- Epithin antibody
- HAI antibody
- Matriptase antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody - Catalytic domain (ab28266)ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab28266 被引用在 5 文献中.
- Marchelletta RR et al. T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling. J Clin Invest 131:N/A (2021). PubMed: 34623320
- Sun P et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). PubMed: 29560101
- Kubyshkin AV et al. THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER. Krim Z Eksp Klin Med 8:11-20 (2018). PubMed: 31131224
- Sun P et al. Decreasing the ratio of matriptase/HAI-1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep 14:1465-74 (2016). WB ; Human . PubMed: 27356668
- Takeichi T et al. Whole-exome sequencing diagnosis of two autosomal recessive disorders in one family. Br J Dermatol 172:1407-11 (2015). PubMed: 25308318