Anti-SSEA3抗体[MC631] (ab16286)
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-316783-anti-ssea3-antibody-mc631-flow-cytometry.jpg "Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)")
Key features and details
- Rat monoclonal [MC631] to SSEA3
- Suitable for: IHC-FoFr, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-SSEA3抗体[MC631] -
描述
大鼠单克隆抗体[MC631] to SSEA3 -
宿主
Rat -
经测试应用
适用于: IHC-FoFr, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 2102Ep Human Embryonic Carcinoma Cells
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常规说明
The monoclonal antibody that defines SSEA-3 was derived by fusing cells of the murine myeloma cell line SP2/0 with the spleen cells of a Fisher rat immunized with 4-8-cell-stage zonapellucida-free embryos from randomly bred mice.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Tissue culture supernatant was cross flow concentrated. -
克隆
单克隆 -
克隆编号
MC631 -
骨髓瘤
P3x63-Ag8.653 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
1/50.
ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-FoFr
Use at an assay dependent concentration. |
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Flow Cyt
1/50. ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter (Shevinsky L. H. et al., 1982, Cell 30:697 and Kannagi R. et al., 1983, J. Biol. Chem. 258:8934). SSEA3 is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes. -
别名
- Stage specific embryonic antigen 3 antibody
图片
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Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of Prof Chris Denning's lab, University of Nottingham
Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-24923-anti-ssea3-antibody-mc631-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of the laboratory of Peter Andrews.The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286. -
![Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)](https://www.abcam.cn/ps/products/16/ab16286/Images/ab16286-24924-anti-ssea3-antibody-mc631-flow-cytometry.jpg)
Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is from an anonymous collaborator.Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab16286 被引用在 39 文献中.
- Yoshimatsu S et al. Step-by-step protocols for non-viral derivation of transgene-free induced pluripotent stem cells from somatic fibroblasts of multiple mammalian species. Dev Growth Differ 64:325-341 (2022). PubMed: 35841539
- Kim G et al. High throughput screening of mesenchymal stem cell lines using deep learning. Sci Rep 12:17507 (2022). PubMed: 36266301
- Jabali A et al. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation. EMBO Rep 23:e54027 (2022). PubMed: 35289477
- Alvarez-Palomo AB et al. A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair, generating improved genetically stable human induced pluripotent stem cells. Stem Cells 39:866-881 (2021). PubMed: 33621399
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080