Anti-SSBP1抗体(ab74710)
Key features and details
- Rabbit polyclonal to SSBP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SSBP1抗体
参阅全部 SSBP1 一抗 -
描述
兔多克隆抗体to SSBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
A synthesized peptide derived from internal sequence of human SSBP1.
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阳性对照
- Human pancreas tissue; extracts from HeLa cells and HUVEC cells; A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74710于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. -
序列相似性
Contains 1 SSB domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 6742 Human
- Entrez Gene: 381760 Mouse
- Entrez Gene: 54304 Rat
- Omim: 600439 Human
- SwissProt: Q04837 Human
- SwissProt: Q9CYR0 Mouse
- SwissProt: P28042 Rat
- Unigene: 490394 Human
see all -
别名
- mitochondrial antibody
- Mt SSB antibody
- Mt-SSB antibody
see all
图片
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Western blot - Anti-SSBP1 antibody (ab74710)All lanes : Anti-SSBP1 antibody (ab74710) at 1/500 dilution
Lane 1 : Extracts from HeLa cells with no peptide
Lane 2 : Extracts from HUVEC cells with no peptide
Lane 3 : Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 26 kDa, 45 kDa. We are unsure as to the identity of these extra bands.Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab74710)Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel: no peptide; right-hand panel: with peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (ab74710)Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel: no peptide; right-hand panel: with peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74710 被引用在 3 文献中.
- Reyes A et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360
- Li Q et al. A functional polymorphism ofSSBP1gene predicts prognosis and response to chemotherapy in resected gastric cancer patients. Oncotarget 8:110861-110876 (2017). PubMed: 29340022
- van Loon B & Samson LD Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB). DNA Repair (Amst) 12:177-87 (2013). PubMed: 23290262