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AB61800

Anti-SSB (phospho S366)抗体

Anti-SSB (phospho S366) antibody

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Rabbit Polyclonal SSB phospho S366 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Lupus La protein phospho S366 aa 300-400.

查看别名

Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB

2 Images
Western blot - Anti-SSB (phospho S366) antibody (AB61800)
  • WB

Unknown

Western blot - Anti-SSB (phospho S366) antibody (AB61800)

All lanes:

Western blot - Anti-SSB (phospho S366) antibody (ab61800) at 1/500 dilution

Lane 1:

extracts from 293 cells at 5 µg

Lane 2:

extracts from 293 cells at 5 µg with immunizing peptide

Predicted band size: 46 kDa

Observed band size: 45 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SSB (phospho S366) antibody (AB61800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SSB (phospho S366) antibody (AB61800)

ab61800 at 1/500 dilution staining HeLa cells, (left) treated with Forskolin (40nM, 30mins), (right) untreated cells.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human Lupus La protein phospho S366 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P05455

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The single-stranded DNA-binding protein (SSB) also known as La protein in humans has a mechanical role in stabilizing single-stranded DNA (ssDNA) during replication recombination and repair processes. In E. coli the SSB protein weighs approximately 19 kDa and is essential for maintaining genome integrity. SSB expression occurs in various organisms with bacterial SSBs being key to prokaryotic cells while homologs like La/SSB in humans are more involved in post-transcriptional processes. Anti-SSB antibodies target this protein often in research and diagnostic applications.
Biological function summary

The single-stranded DNA-binding protein plays an important role in the orchestration of DNA metabolism. It binds to ssDNA substrates acting as a scaffold for recruiting other proteins and it frequently involves itself in complex formation with other cellular factors such as replication proteins. This function proves important in stabilizing unwound DNA and regulating access for proteins involved in DNA synthesis and repair. Anti-La SSB antibodies are typically used to study aberrant RNA processing events.

Pathways

The SSB protein influences the DNA replication and repair pathways. SSB's interaction with proteins like DNA polymerase and exonuclease suggests its cooperation with these enzymes to ensure precise DNA synthesis and to facilitate proper repair of DNA. Anti-SSB Ab can be used to investigate specific pathway dysregulations providing insights into pathway alterations that impact genome stability. Notably SSB's role in coordinating with other repair proteins emphasizes its centrality in maintaining steady state of DNA integrity.

The single-stranded DNA-binding protein has vital connections to autoimmune diseases. Sjögren's syndrome and systemic lupus erythematosus often see the presence of anti-SSB antibodies indicating an autoimmune response against this protein. These disorders associate with the human La protein an autoimmune target in these disease states. Understanding SSB's interaction with other proteins such as Ro60 in these conditions gives insights into disease pathology and potential areas for therapeutic intervention.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed : 2470590, PubMed : 3192525). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed : 12384597).
See full target information Lupus La protein phospho S366
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