重组Anti-SSB抗体[EPR6570] (ab124932)
![Western blot - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.cn/ps/products/124/ab124932/Images/ab124932-138962-anti-ssb-antibody-epr6570-western-blot.jpg "Western blot - Anti-SSB antibody [EPR6570] (ab124932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6570] to SSB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SSB抗体[EPR6570]
参阅全部 SSB 一抗 -
描述
兔单克隆抗体[EPR6570] to SSB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SSB aa 100-200. The exact sequence is proprietary.
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阳性对照
- Jurkat, K562, HeLa, Ramos, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human colon tissue; Human thyroid adenocarcinoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 4.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6570 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124932于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
序列相似性
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6741 Human
- Entrez Gene: 20823 Mouse
- Entrez Gene: 81783 Rat
- GenBank: NP_003133.1 Human
- Omim: 109090 Human
- SwissProt: P05455 Human
- SwissProt: P32067 Mouse
- SwissProt: P38656 Rat
see all -
别名
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
see all
图片
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Western blot - Anti-SSB antibody [EPR6570] (ab124932)All lanes : Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : C6 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.cn/ps/products/124/ab124932/Images/ab124932-138961-anti-ssb-antibody-epr6570-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)Formalin-fixed, paraffin-embedded human colon tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.cn/ps/products/124/ab124932/Images/ab124932-138960-anti-ssb-antibody-epr6570-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody [EPR6570] (ab124932)Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.cn/ps/products/124/ab124932/Images/ab124932-138959-anti-ssb-antibody-epr6570.jpg)
OI-RD Scanning - Anti-SSB antibody [EPR6570] (ab124932) -
![Anti-SSB antibody [EPR6570] (ab124932)](https://www.abcam.cn/ps/products/124/ab124932/Images/ab124932-5-benefits-of-recombinant-antibodies.png)
Anti-SSB antibody [EPR6570] (ab124932)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124932 被引用在 1 文献中.
- Huang X et al. La protein regulates protein expression by binding with the mRNAs of target genes and participates the pathological process of ovarian cancer. Front Oncol 12:763480 (2022). PubMed: 36110943