Anti-SRSF3抗体(ab125124)
Key features and details
- Rabbit polyclonal to SRSF3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SRSF3抗体
参阅全部 SRSF3 一抗 -
描述
兔多克隆抗体to SRSF3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SRSF3 (C terminal).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+, Ca2+) -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
|
| IHC-P | (2) |
Use a concentration of 5 µg/ml.
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| ICC/IF |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. |
|
ICC/IF
1/100 - 1/500. |
靶标
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功能
May be involved in RNA processing in relation with cellular proliferation and/or maturation. -
序列相似性
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Extensively phosphorylated on serine residues in the RS domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6428 Human
- Omim: 603364 Human
- SwissProt: P84103 Human
- Unigene: 405144 Human
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别名
- arginine/serine-rich 3 antibody
- Pre mRNA splicing factor SRP20 antibody
- Pre-mRNA-splicing factor SRP20 antibody
see all
图片
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Western blot - Anti-SRSF3 antibody (ab125124)This image is courtesy of Suypriya Sen
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.
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Western blot - Anti-SRSF3 antibody (ab125124)All lanes : Anti-SRSF3 antibody (ab125124) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : Jurkat cell extract
Lane 3 : Jurkat cell extract with synthesized peptide
Predicted band size: 19 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab125124 被引用在 5 文献中.
- Xu XC et al. SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1. Cell Death Dis 14:511 (2023). PubMed: 37558679
- Simsek D et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
- Lin YJ et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). PubMed: 26048696
- Ajiro M et al. Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates. Int J Biol Sci 11:1248-56 (2015). PubMed: 26435690
- Sen S et al. Deletion of splicing factor SRSF3 in hepatocytes predisposes to hepatocellular carcinoma in mice. Hepatology N/A:N/A (2014). Mouse . PubMed: 25132062