AB125124

Anti-SRSF3抗体

Name: SRSF3抗体 (ab125124)| Abcam中文官网
Brand: Abcam Limited
SKU: AB125124
Price: 4767 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-SRSF3 antibody

(3 Reviews)

(5 Publications)

Rabbit Polyclonal SRSF3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF3.

查看别名

SFRS3, SRP20, SRSF3, Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

Unknown

Western blot - Anti-SRSF3 antibody (AB125124)

All lanes:

Western blot - Anti-SRSF3 antibody (ab125124) at 1/500 dilution

Lane 1:

HeLa cell extract

Lane 2:

Jurkat cell extract

Lane 3:

Jurkat cell extract with synthesized peptide

Predicted band size: 19 kDa

false

AbReview35828****

Western blot - Anti-SRSF3 antibody (AB125124)

All lanes:

Western blot - Anti-SRSF3 antibody (ab125124) at 1/2000 dilution

Lanes 1 - 2:

SRSF3 knock-out Mouse hepatocyte cell lysate at 25 µg

Lanes 3 - 4:

Wild type Mouse hepatocyte cell lysate at 25 µg

Secondary

All lanes:

Anti-rabbit IgG polyclonal HRP conjugate at 1/5000 dilution

Predicted band size: 19 kDa

false

Exposure time: 5s

This image is courtesy of Suypriya Sen

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human SRSF3. The exact immunogen used to generate this antibody is proprietary information.

P84103

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed : 17036044, PubMed : 26876937, PubMed : 32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed : 26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed : 11336712, PubMed : 18364396, PubMed : 28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed : 11336712, PubMed : 18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1 : interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).

See full target information SRSF3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:511 PubMed37558679

2023

SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Chen Xu,Jia-Xin Jiang,Ya-Qing Zhou,Shuai He,Yang Liu,Yi-Qi Li,Pan-Pan Wei,Jin-Xin Bei,Jian Sun,Chun-Ling Luo

Cell 169:1051-1065.e18 PubMed28575669

2017

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria Barna

International journal of biological sciences 11:1248-56 PubMed26435690

2015

Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiko Ajiro,Rong Jia,Rui-Hong Wang,Chu-Xia Deng,Zhi-Ming Zheng

BioMedicine 5:10 PubMed26048696

2015

P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lin,Tsung-Jung Ho,Ting-Hsu Lin,Wei-Yi Hsu,Shao-Mei Huang,Chiu-Chu Liao,Chih-Ho Lai,Xiang Liu,Hsinyi Tsang,Chien-Chen Lai,Fuu-Jen Tsai

Hepatology (Baltimore, Md.) 61:171-83 PubMed25132062

2014

Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice.

Applications

Unspecified application

Species

Mouse

Supriya Sen,Magda Langiewicz,Hassan Jumaa,Nicholas J G Webster

View all publications

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Anti-SRSF3抗体

Anti-SRSF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

Western blot - Anti-SRSF3 antibody (AB125124)

Western blot - Anti-SRSF3 antibody (AB125124)