AB200199

重组Anti-SRP72抗体[EPR17143]

Anti-SRP72 antibody [EPR17143]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal SRP72 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

查看别名

Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein

4 Images

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SRP72 with ab200199 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution

All lanes:

Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 75 kDa,89 kDa

Observed band size: 75 kDa,89 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17143

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (PubMed : 34020957). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER (PubMed : 34020957). The SRP complex targets the ribosome-nascent chain complex to the SRP receptor (SR), which is anchored in the ER, where SR compaction and GTPase rearrangement drive cotranslational protein translocation into the ER (PubMed : 34020957). Binds the signal recognition particle RNA (7SL RNA) in presence of SRP68 (PubMed : 21073748, PubMed : 27899666). Can bind 7SL RNA with low affinity (PubMed : 21073748, PubMed : 27899666). The SRP complex possibly participates in the elongation arrest function (By similarity).

See full target information SRP72

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012766 PubMed39621795

2024

Flaviviruses induce ER-specific remodelling of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Him Wong,Dorian Richard Kenneth Crudgington,Lewis Siu,Sumana Sanyal

International journal of molecular sciences 22: PubMed34066088

2021

Alternative C3 Complement System: Lipids and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maisa Garcia-Arguinzonis,Elisa Diaz-Riera,Esther Peña,Rafael Escate,Oriol Juan-Babot,Pedro Mata,Lina Badimon,Teresa Padro

Molecular cell 75:1103-1116.e9 PubMed31420216

2019

Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.

Applications

Unspecified application

Species

Unspecified reactive species

Dianrong Li,Jie Chen,Youwei Ai,Xiaoqiong Gu,Li Li,Di Che,Zhaodi Jiang,Lin Li,She Chen,Huangwei Huang,Jiawen Wang,Tao Cai,Yang Cao,Xiangbin Qi,Xiaodong Wang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SRP72抗体[EPR17143]

Anti-SRP72 antibody [EPR17143]

Flow Cytometry (Intracellular) - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

Western blot - Anti-SRP72 antibody [EPR17143] (AB200199)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原