重组Anti-SRP19抗体[EPR8378] (ab131239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8378] to SRP19
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SRP19抗体[EPR8378]
参阅全部 SRP19 一抗 -
描述
兔单克隆抗体[EPR8378] to SRP19 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SRP19 aa 1-100. The exact sequence is proprietary.
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阳性对照
- Jurkat, HeLa, Daudi, and HepG2 cell lysates; Human spleen tissue; HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 4.10 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8378 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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IP |
Use at an assay dependent concentration.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
IP
Use at an assay dependent concentration. |
靶标
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功能
Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. -
序列相似性
Belongs to the SRP19 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6728 Human
- Entrez Gene: 66384 Mouse
- Entrez Gene: 291685 Rat
- Omim: 182175 Human
- SwissProt: P09132 Human
- SwissProt: Q9D7A6 Mouse
- Unigene: 637001 Human
- Unigene: 29452 Mouse
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别名
- Signal recognition particle 19 kDa protein antibody
- signal recognition particle 19kDa antibody
- SRP19 antibody
- SRP19_HUMAN antibody
图片
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All lanes : Anti-SRP19 antibody [EPR8378] (ab131239) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP19 antibody [EPR8378] (ab131239)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling SRP19 with ab131239 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HepG2 cells labelling SRP19 with ab131239 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131239 被引用在 1 文献中.
- Li D et al. Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. Mol Cell N/A:N/A (2019). PubMed: 31420216