Anti-SRGAP1抗体(ab76926)
Key features and details
- Rabbit polyclonal to SRGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SRGAP1抗体
参阅全部 SRGAP1 一抗 -
描述
兔多克隆抗体to SRGAP1 -
宿主
Rabbit -
特异性
Does not crossreact with human srGAP2 or human srGAP3 -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 979 and 1029 of human SRGAP1 (NP_065813.1)
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab76926 was affinity purified using an epitope specific to SRGAP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42. -
组织特异性
Expressed in brain, lung, kidney, and testis. -
序列相似性
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
结构域
The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions. - Information by UniProt
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数据库链接
- Entrez Gene: 57522 Human
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- Unigene: 210751 Human
- Unigene: 593803 Human
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别名
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
see all
图片
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Western blot - Anti-SRGAP1 antibody (ab76926)All lanes : Anti-SRGAP1 antibody (ab76926) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Lane 4 : 293T whole cell lysate. at 50 µg
Predicted band size: 124 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-SRGAP1 antibody (ab76926)ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab76926 被引用在 2 文献中.
- Chiang TS et al. ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation. Mol Biol Cell 30:69-81 (2019). PubMed: 30427759
- Genet G et al. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nat Commun 10:2350 (2019). PubMed: 31138815