Anti-SREBP1抗体(ab28481)
Key features and details
- Rabbit polyclonal to SREBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SREBP1抗体
参阅全部 SREBP1 一抗 -
描述
兔多克隆抗体to SREBP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
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阳性对照
- ICC/IF: Human HepG2 cells, mouse NIH-3T3, C2C12 cells; WB: mouse and rat liver, MCF-7 and MDA-MB-231 cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
1/50 - 1/500.
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| WB | (4) |
1/500 - 1/5000.
Detects an ~68 and 120 kDa protein representing SREBP1 in mouse and rat liver samples as well as rat kidney samples. A predominant band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. |
| 说明 |
|---|
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ICC/IF
1/50 - 1/500. |
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WB
1/500 - 1/5000. Detects an ~68 and 120 kDa protein representing SREBP1 in mouse and rat liver samples as well as rat kidney samples. A predominant band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. |
靶标
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功能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). -
组织特异性
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. -
序列相似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. -
细胞定位
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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数据库链接
- Entrez Gene: 6720 Human
- Entrez Gene: 20787 Mouse
- Entrez Gene: 78968 Rat
- Omim: 184756 Human
- SwissProt: P36956 Human
- SwissProt: Q9WTN3 Mouse
- SwissProt: P56720 Rat
- Unigene: 592123 Human
see all -
别名
- ADD 1 antibody
- bHLHd1 antibody
- Class D basic helix-loop-helix protein 1 antibody
see all
图片
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Western blot - Anti-SREBP1 antibody (ab28481)All lanes : Anti-SREBP1 antibody (ab28481) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Developed using the ECL technique. -
Western blot - Anti-SREBP1 antibody (ab28481)All lanes : Anti-SREBP1 antibody (ab28481) at 1/1000 dilution
Lane 1 : MDA-MB-231 cell lysate with Fat-free milk / PBST
Lane 2 : MCF-7 cell lysate with Fat-free milk / PBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 120 kDa why is the actual band size different from the predicted?Western blot analysis on a 4-8% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SREBP1 antibody (ab28481)Immunocytochemical analysis of formalin-fixed HepG2 cells (human liver hepatocellular carcinoma cell line) using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains. -
Immunocytochemistry/ Immunofluorescence - Anti-SREBP1 antibody (ab28481)Immunocytochemical analysis of formalin-fixed C2C12 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification was 60X
The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains. -
Immunocytochemistry/ Immunofluorescence - Anti-SREBP1 antibody (ab28481)Immunocytochemical analysis of formalin-fixed NIH 3T3 cell lines using immunofluorescence to label SREBP1 with ab28481 at a concentration of 1/100 in 3% BSA-PBS and incubated overnight in a humid environment at 4°C. Prior to labelling, cells were permeablised with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, they were subsequently blocked with 3% BSA-PBS for 30 minutes at room temperature. The secondary used was a DyLight® conjugate (green) and was incubated at room temperature in the dark. The cells were counterstained with DAPI against DNA labelling the nucear compartments blue and a red fluorescent phalloidin stain against F-Actin. Magnification is 60X
The left image is a negative control in the absence of ab28481, the right image is in the prescence of ab28481, the secondary and counterstains.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (151)
ab28481 被引用在 151 文献中.
- Zheng H et al. Dietary chenodeoxycholic acid attenuates high-fat diet-induced growth retardation, lipid accumulation and bile acid metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco. Br J Nutr 131:921-934 (2024). PubMed: 37905695
- Deng GH et al. Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease. Mil Med Res 10:53 (2023). PubMed: 37941054
- Ma P et al. Lanosterol Synthase Prevents EMT During Lens Epithelial Fibrosis Via Regulating SREBP1. Invest Ophthalmol Vis Sci 64:12 (2023). PubMed: 38079167
- Liu J et al. Farnesyl diphosphate synthase exacerbates nonalcoholic steatohepatitis via the activation of AHR-CD36 axis. FASEB J 37:e23035 (2023). PubMed: 37310396
- Xu J et al. Replacement of Dietary Fishmeal with Clostridium autoethanogenum Protein on Lipidomics and Lipid Metabolism in Muscle of Pearl Gentian Grouper. Aquac Nutr 2023:6723677 (2023). PubMed: 37424881